• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

  • Featured Product
  • KD/KO Validated

PLAA Polyclonal antibody

PLAA Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF, IP, ELISA

Conjugate

Unconjugated

Cat no : 12529-1-AP

Synonyms

DOA1, PLA2P, PLAA, PLAP

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of MCF-7 cells using PLAA Polyclonal antibody (12529-1-AP)

    WB analysis of MCF-7 using 12529-1-AP

    WB result of PLAA antibody (12529-1-AP; 1:1200; incubated at room temperature for 1.5 hours) with sh-Control and sh-PLAA transfected MCF-7 cells.

  • Western Blot (WB) analysis of K-562 cells using PLAA Polyclonal antibody (12529-1-AP)

    WB analysis of K-562 using 12529-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 12529-1-AP (PLAA antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using PLAA Polyclonal antibody (12529-1-AP)

    WB analysis of MCF-7 using 12529-1-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 12529-1-AP (PLAA antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of K-562 cells using PLAA Polyclonal antibody (12529-1-AP)

    IP experiment of K-562 using 12529-1-AP

    IP result of anti-PLAA (IP:12529-1-AP, 4ug; Detection:12529-1-AP 1:600) with K-562 cells lysate 3200ug.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using PLAA Polyclonal antibody (12529-1-AP)

    IHC staining of human lung cancer using 12529-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12529-1-AP (PLAA Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using PLAA Polyclonal antibody (12529-1-AP)

    IHC staining of human lung cancer using 12529-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12529-1-AP (PLAA Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human kidney tissue using PLAA Polyclonal antibody (12529-1-AP)

    IHC staining of human kidney using 12529-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 12529-1-AP (PLAA Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using PLAA Polyclonal antibody (12529-1-AP)

    IHC staining of human kidney using 12529-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 12529-1-AP (PLAA Antibody) at dilution of 1:200 (under 40x lens).

☆大好評トライアルサイズ ¥20,000～！ >>> 一次抗体特別価格キャンペーン開催中！

Tested Applications

Positive WB detected inK-562 cells, MCF-7 cells
Positive IP detected inK-562 cells
Positive IHC detected inhuman lung cancer tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12529-1-AP targets PLAA in WB, IHC, IF, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PLAA fusion protein Ag3224 相同性解析による交差性が予測される生物種
Full Name phospholipase A2-activating protein
Calculated molecular weight 738 aa, 81 kDa
Observed molecular weight 72-74 kDa
GenBank accession numberBC032551
Gene symbol PLAA
Gene ID (NCBI) 9373
RRIDAB_2164881
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Phospholipase A-2-activating protein (PLAA), also named PLAP, can interact with ubiquitin and is involved in the maintenance of ubiquitin levels. PLAA contains multiple consensus domains including WD repeats, PFU domain and PUL domain. Although the function of PLAA remains unclear, ubiquitin binding of PLAA might be the central role that connects ubiquitination and degradation in ERAD. Recent finding revealed that PLAA served as a novel nociveptive mediator after incision, and its expression level is regulated by miR-203.

Protocols

Product Specific Protocols
WB protocol for PLAA antibody 12529-1-APDownload protocol
IHC protocol for PLAA antibody 12529-1-APDownload protocol
IP protocol for PLAA antibody 12529-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB, IHC

Anesthesiology

miR-203 regulates nociceptive sensitization after incision by controlling phospholipase A2 activating protein expression.

Authors - Sun Yuan Y
View Article
humanWB,IF

Biochim Biophys Acta Mol Cell Res

Phospholipase A2-activating protein induces mitophagy trough anti-apoptotic MCL1-mediated NLRX1 oligomerization

Authors - Zhilong Zheng
  • KD Validated
View Article