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Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using PLAP Monoclonal antibody (60294-1-Ig)

    WB analysis of HepG2 using 60294-1-Ig (same clone as 60294-1-PBS)

    WB result of PLAP antibody (60294-1-Ig, 1:1000) with si-control and si-PLAP transfected HepG2 cells. This data was developed using the same antibody clone with 60294-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using PLAP Monoclonal antibody (60294-1-Ig)

    WB analysis of HepG2 using 60294-1-Ig (same clone as 60294-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 60294-1-Ig (PLAP antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60294-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using PLAP Monoclonal antibody (60294-1-Ig)

    WB analysis using 60294-1-Ig (same clone as 60294-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 60294-1-Ig (PLAP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60294-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human placenta tissue using PLAP Monoclonal antibody (60294-1-Ig)

    WB analysis of human placenta using 60294-1-Ig (same clone as 60294-1-PBS)

    human placenta tissue were subjected to SDS PAGE followed by western blot with 60294-1-Ig (PLAP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60294-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human placenta tissue using PLAP Monoclonal antibody (60294-1-Ig)

    WB analysis of human placenta using 60294-1-Ig (same clone as 60294-1-PBS)

    human placenta tissue were subjected to SDS PAGE followed by western blot with 60294-1-Ig (PLAP antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60294-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human appendicitis tissue using PLAP Monoclonal antibody (60294-1-Ig)

    IHC staining of human appendicitis using 60294-1-Ig (same clone as 60294-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 60294-1-Ig (PLAP antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60294-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human appendicitis tissue using PLAP Monoclonal antibody (60294-1-Ig)

    IHC staining of human appendicitis using 60294-1-Ig (same clone as 60294-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 60294-1-Ig (PLAP antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60294-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human malignant melanoma tissue using PLAP Monoclonal antibody (60294-1-Ig)

    IHC staining of human malignant melanoma using 60294-1-Ig (same clone as 60294-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 60294-1-Ig (PLAP antibody) at dilution of 1:4000 (under 10x lens). This data was developed using the same antibody clone with 60294-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human malignant melanoma tissue using PLAP Monoclonal antibody (60294-1-Ig)

    IHC staining of human malignant melanoma using 60294-1-Ig (same clone as 60294-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 60294-1-Ig (PLAP antibody) at dilution of 1:4000 (under 40x lens). This data was developed using the same antibody clone with 60294-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using PLAP Monoclonal antibody (60294-1-Ig)

    IHC staining of human placenta using 60294-1-Ig (same clone as 60294-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 60294-1-Ig (PLAP antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60294-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using PLAP Monoclonal antibody (60294-1-Ig)

    IHC staining of human placenta using 60294-1-Ig (same clone as 60294-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 60294-1-Ig (PLAP antibody at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 60294-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using PLAP Monoclonal antibody (60294-1-Ig)

    IHC staining of human placenta using 60294-1-Ig (same clone as 60294-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 60294-1-Ig (PLAP antibody at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 60294-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

PLAP Monoclonal antibody, PBS Only

PLAP Monoclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 60294-1-PBS
Clone No.2B7C9

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Synonyms

PLAP-1, PALP, EC:3.1.3.1, ALPP, ALP

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

60294-1-PBS targets PLAP in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen PLAP fusion protein Ag13361 相同性解析による交差性が予測される生物種
Full Name alkaline phosphatase, placental (Regan isozyme)
Calculated molecular weight 58 kDa
Observed molecular weight 66 kDa
GenBank accession numberBC009647
Gene Symbol PLAP
Gene ID (NCBI) 250
RRIDAB_2881409
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP05187
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Placental alkaline phosphatase (PLAP) is a glycosyl phosphatidylinositol (GPI)-anchored sialoglycoprotein , which shows greater resistance to heat inactivation than other alkaline phosphatase. Presented at high levels in placental trophoblasts, PLAP is recognized as an important marker of differentiation in human malignancies.