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uPA/Urokinase Polyclonal antibody

Name: uPA/Urokinase Polyclonal antibody
Brand: Proteintech
SKU: 17968-1-AP

uPA/Urokinase Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 17968-1-AP

Synonyms

PLAU, UPA, U plasminogen activator, u PA, EC:3.4.21.73

Validation Data Gallery

Filter:

AllWBIPIHCIF/ICC

WB analysis using 17968-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 17968-1-AP (uPA/Urokinase antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
WB analysis of human brain using 17968-1-AP
human brain tissue were subjected to SDS PAGE followed by western blot with 17968-1-AP (uPA antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of A375 using 17968-1-AP
A375 cells were subjected to SDS PAGE followed by western blot with 17968-1-AP (uPA antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IP experiment of HeLa using 17968-1-AP
IP result of anti-uPA/Urokinase (IP:17968-1-AP, 4ug; Detection:17968-1-AP 1:1000) with HeLa cells lysate 2800ug.
IHC staining of human breast cancer using 17968-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17968-1-AP (uPA/Urokinase antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 17968-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17968-1-AP (uPA/Urokinase antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human bowen disease using 17968-1-AP
Immunohistochemical analysis of paraffin-embedded human bowen disease slide using 17968-1-AP (uPA/Urokinase antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human ovary using 17968-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary using 17968-1-AP (uPA antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human ovary using 17968-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary using 17968-1-AP (uPA antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human skin using 17968-1-AP
Immunohistochemical analysis of paraffin-embedded human skin using 17968-1-AP (uPA antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human skin using 17968-1-AP
Immunohistochemical analysis of paraffin-embedded human skin using 17968-1-AP (uPA antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human lung using 17968-1-AP
Immunohistochemical analysis of paraffin-embedded human lung using 17968-1-AP (uPA antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human lung using 17968-1-AP
Immunohistochemical analysis of paraffin-embedded human lung using 17968-1-AP (uPA antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human prostate cancer using 17968-1-AP
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 17968-1-AP (uPA/Urokinase antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human prostate cancer using 17968-1-AP
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 17968-1-AP (uPA/Urokinase antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HeLa using 17968-1-AP
Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using uPA/Urokinase antibody (17968-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Cat No. 17968-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	DU 145 cells, human brain tissue, A375 cells, Hela cells, LNCaP cells
Positive IP detected in	HeLa cells
Positive IHC detected in	human breast cancer tissue, human bowen disease, human lung tissue, human ovary tissue, human prostate cancer tissue, human skin tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:8000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 34 publications below
IHC	See 11 publications below
IF	See 6 publications below
IP	See 1 publications below
CoIP	See 1 publications below

Product Information

17968-1-AP targets uPA/Urokinase in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat, bovine
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	uPA/Urokinase fusion protein Ag11813 相同性解析による交差性が予測される生物種
Full Name	plasminogen activator, urokinase
Calculated molecular weight	431 aa, 49 kDa
Observed molecular weight	54 kDa
GenBank accession number	BC013575
Gene symbol	Urokinase
Gene ID (NCBI)	5328
RRID	AB_2165202
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

PLAU(Urokinase-type plasminogen activator) is also named as uPA and belongs to the peptidase S1 family.UPA initiates a proteolytic cascade, which degrades extracellular matrix during tissue growth and remodelling.The secreted proform of UPA is activated after binding to a specific high affinity cell surface receptor. Secreted UPA is exposed to rapid inactivation in the pericellular space by plasminogen activator inhibitor-1 (PAI-1), which is stored in the extracellular matrix bound to vitronectin(PMID:9665342).It has 2 isoforms produced by alternative splicing. Defects in PLAU are the cause of Quebec platelet disorder (QPD).

Protocols

Product Specific Protocols
WB protocol for uPA/Urokinase antibody 17968-1-AP	Download protocol
IHC protocol for uPA/Urokinase antibody 17968-1-AP	Download protocol
IF protocol for uPA/Urokinase antibody 17968-1-AP	Download protocol
IP protocol for uPA/Urokinase antibody 17968-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Sci Adv p53 loss activates prometastatic secretory vesicle biogenesis in the Golgi. Authors - Xiaochao Tan View Article
mouse	WB	Gastroenterology Inhibition of Plasmin Protects Against Colitis in Mice by Suppressing Matrix Metalloproteinase 9-mediated Cytokine Release From Myeloid Cells. Authors - Shinya Munakata View Article
mouse	IF, WB	J Exp Med PDGF-D activation by macrophage-derived uPA promotes AngII-induced cardiac remodeling in obese mice. Authors - Yu-Wen Cheng View Article
human	IHC	Aging (Albany NY) Tertiary lymphoid structure stratifies glioma into three distinct tumor subtypes. Authors - Xingwang Zhou View Article
mouse	WB	Angiogenesis PDGFD switches on stem cell endothelial commitment. Authors - Weisi Lu View Article
human	WB	Aging (Albany NY) NFAT5 directs hyperosmotic stress-induced fibrin deposition and macrophage infiltration via PAI-1 in endothelium. Authors - Pingping Ma View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

uPA/Urokinase Polyclonal antibody

uPA/Urokinase Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Validation Data Gallery

WB analysis using 17968-1-AP

WB analysis of human brain using 17968-1-AP

WB analysis of A375 using 17968-1-AP

IP experiment of HeLa using 17968-1-AP

IHC staining of human breast cancer using 17968-1-AP

IHC staining of human breast cancer using 17968-1-AP

IHC staining of human bowen disease using 17968-1-AP

IHC staining of human ovary using 17968-1-AP

IHC staining of human ovary using 17968-1-AP

IHC staining of human skin using 17968-1-AP

IHC staining of human skin using 17968-1-AP

IHC staining of human lung using 17968-1-AP

IHC staining of human lung using 17968-1-AP

IHC staining of human prostate cancer using 17968-1-AP

IHC staining of human prostate cancer using 17968-1-AP

IF Staining of HeLa using 17968-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

p53 loss activates prometastatic secretory vesicle biogenesis in the Golgi.

Inhibition of Plasmin Protects Against Colitis in Mice by Suppressing Matrix Metalloproteinase 9-mediated Cytokine Release From Myeloid Cells.

PDGF-D activation by macrophage-derived uPA promotes AngII-induced cardiac remodeling in obese mice.

Tertiary lymphoid structure stratifies glioma into three distinct tumor subtypes.

PDGFD switches on stem cell endothelial commitment.

NFAT5 directs hyperosmotic stress-induced fibrin deposition and macrophage infiltration via PAI-1 in endothelium.