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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PLB1 Polyclonal antibody

PLB1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 26835-1-AP

Synonyms

Phospholipase A2, Lysophospholipase, hPLB, EC:3.1.1.5, EC:3.1.1.4

Validation Data Gallery

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  • Western Blot (WB) analysis of DU 145 cells using PLB1 Polyclonal antibody (26835-1-AP)

    WB analysis of DU 145 using 26835-1-AP

    DU 145 cells were subjected to SDS PAGE followed by western blot with 26835-1-AP (PLB1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human prostate hyperplasia tissue using PLB1 Polyclonal antibody (26835-1-AP)

    IHC staining of human prostate hyperplasia using 26835-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 26835-1-AP (PLB1 Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human prostate hyperplasia tissue using PLB1 Polyclonal antibody (26835-1-AP)

    IHC staining of human prostate hyperplasia using 26835-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 26835-1-AP (PLB1 Antibody) at dilution of 1:200 (under 40x lens).

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Tested Applications

Positive WB detected inDU 145 cells
Positive IHC detected inhuman prostate hyperplasia tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:100-1:400
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

26835-1-AP targets PLB1 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PLB1 fusion protein Ag25240 相同性解析による交差性が予測される生物種
Full Name phospholipase B1
Observed molecular weight 45 kDa
GenBank accession numberBC150633
Gene symbol PLB1
Gene ID (NCBI) 151056
RRIDAB_2880652
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for PLB1 antibody 26835-1-APDownload protocol
IHC protocol for PLB1 antibody 26835-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols