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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PLCB2 Polyclonal antibody

PLCB2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 27456-1-AP

Synonyms

PLC beta 2, Phospholipase C-beta-2, Phospholipase C beta 2, Phosphoinositide phospholipase C-beta-2, EC:3.1.4.11

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PLCB2 Polyclonal antibody (27456-1-AP)

    WB analysis using 27456-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27456-1-AP (PLCB2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using PLCB2 Polyclonal antibody (27456-1-AP)

    WB analysis using 27456-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27456-1-AP (PLCB2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using PLCB2 Polyclonal antibody (27456-1-AP)

    IHC staining of mouse liver using 27456-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 27456-1-AP (PLCB2 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of THP-1 cells using PLCB2 Polyclonal antibody (27456-1-AP)

    IF Staining of THP-1 using 27456-1-AP

    Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using PLCB2 antibody (27456-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Tested Applications

Positive WB detected inTHP-1 cells, HL-60 cells
Positive IHC detected inmouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inTHP-1 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:300-1:1200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

27456-1-AP targets PLCB2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PLCB2 fusion protein Ag25771 相同性解析による交差性が予測される生物種
Full Name phospholipase C, beta 2
Calculated molecular weight 134 kDa
Observed molecular weight 134 kDa
GenBank accession numberBC000939
Gene symbol PLCB2
Gene ID (NCBI) 5330
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PLCB2 (1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-2) is also named as PLC-beta-2. PLCB2 is a critical regulator of platelet responses upon activation (PMID: 27465150). NF-κB regulates expression of PLC-β2. The PLCB2 genes was found to be differentially expressed in human breast cancer MCF-7 cells, and to be associated with multidrug resistance (PMID: 2951275). The knockdown of PLCB2 suppressed cell viability and promoted cell apoptosis by activating the Ras/Raf/MAPK pathway. Thus, PLCB2 may utilized as a potential therapeutic target in patients with melanoma (PMID: 31746389).

Protocols

Product Specific Protocols
WB protocol for PLCB2 antibody 27456-1-APDownload protocol
IHC protocol for PLCB2 antibody 27456-1-APDownload protocol
IF protocol for PLCB2 antibody 27456-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols