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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PLCB3 Polyclonal antibody

PLCB3 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 21370-1-AP

Synonyms

Phospholipase C beta 3, PLC beta 3, PLCB3

Validation Data Gallery

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  • Western Blot (WB) analysis of MCF-7 cells using PLCB3 Polyclonal antibody (21370-1-AP)

    WB analysis of MCF-7 using 21370-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 21370-1-AP (PLCB3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of NIH/3T3 cells using PLCB3 Polyclonal antibody (21370-1-AP)

    WB analysis of NIH/3T3 using 21370-1-AP

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 21370-1-AP (PLCB3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293T cells using PLCB3 Polyclonal antibody (21370-1-AP)

    WB analysis of HEK-293T using 21370-1-AP

    HEK-293T cells were subjected to SDS PAGE followed by western blot with 21370-1-AP (PLCB3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of COLO 320 cells using PLCB3 Polyclonal antibody (21370-1-AP)

    WB analysis of COLO 320 using 21370-1-AP

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 21370-1-AP (PLCB3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A431 cells using PLCB3 Polyclonal antibody (21370-1-AP)

    WB analysis of A431 using 21370-1-AP

    A431 cells were subjected to SDS PAGE followed by western blot with 21370-1-AP (PLCB3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human colon tissue using PLCB3 Polyclonal antibody (21370-1-AP)

    IHC staining of human colon using 21370-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 21370-1-AP (PLCB3 Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human colon tissue using PLCB3 Polyclonal antibody (21370-1-AP)

    IHC staining of human colon using 21370-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 21370-1-AP (PLCB3 Antibody) at dilution of 1:200 (under 40x lens).

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Tested Applications

Positive WB detected inMCF-7 cells, A431 cells, COLO 320 cells, HEK-293T cells, NIH/3T3 cells
Positive IHC detected inhuman colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 21370-1-AP is PLCB3 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PLCB3 fusion protein Ag15845 相同性解析による交差性が予測される生物種
Full Name phospholipase C, beta 3 (phosphatidylinositol-specific)
Calculated molecular weight 1234 aa, 139 kDa
Observed molecular weight 150 kDa
GenBank accession numberBC142681
Gene symbol PLCB3
Gene ID (NCBI) 5331
RRIDAB_2878848
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for PLCB3 antibody 21370-1-APDownload protocol
IHC protocol for PLCB3 antibody 21370-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols