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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PLCB3 Monoclonal antibody (66668-1-Ig)

    WB analysis using 66668-1-Ig (same clone as 66668-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66668-1-Ig (PLCB3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control. This data was developed using the same antibody clone with 66668-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using PLCB3 Monoclonal antibody (66668-1-Ig)

    WB analysis using 66668-1-Ig (same clone as 66668-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66668-1-Ig (PLCB3 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66668-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Sp2/0 cells using PLCB3 Monoclonal antibody (66668-1-Ig)

    WB analysis of Sp2/0 using 66668-1-Ig (same clone as 66668-1-PBS)

    Sp2/0 cells were subjected to SDS PAGE followed by western blot with 66668-1-Ig (PLCB3 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66668-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using PLCB3 Monoclonal antibody (66668-1-Ig)

    WB analysis using 66668-1-Ig (same clone as 66668-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66668-1-Ig (PLCB3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66668-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Jurkat cells using PLCB3 Monoclonal antibody (66668-1-Ig)

    WB analysis of Jurkat using 66668-1-Ig (same clone as 66668-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 66668-1-Ig (PLCB3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66668-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HSC-T6 cells using PLCB3 Monoclonal antibody (66668-1-Ig)

    WB analysis of HSC-T6 using 66668-1-Ig (same clone as 66668-1-PBS)

    HSC-T6 cells were subjected to SDS PAGE followed by western blot with 66668-1-Ig (PLCB3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66668-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of NIH/3T3 cells using PLCB3 Monoclonal antibody (66668-1-Ig)

    WB analysis of NIH/3T3 using 66668-1-Ig (same clone as 66668-1-PBS)

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66668-1-Ig (PLCB3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66668-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using PLCB3 Monoclonal antibody (66668-1-Ig)

    IHC staining of human breast cancer using 66668-1-Ig (same clone as 66668-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66668-1-Ig (PLCB3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66668-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using PLCB3 Monoclonal antibody (66668-1-Ig)

    IHC staining of human breast cancer using 66668-1-Ig (same clone as 66668-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66668-1-Ig (PLCB3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66668-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using PLCB3 Monoclonal antibody (66668-1-Ig)

    IHC staining of human colon using 66668-1-Ig (same clone as 66668-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66668-1-Ig (PLCB3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66668-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using PLCB3 Monoclonal antibody (66668-1-Ig)

    IHC staining of human colon using 66668-1-Ig (same clone as 66668-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66668-1-Ig (PLCB3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66668-1-PBS in a different storage buffer formulation.

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PLCB3 Monoclonal antibody, PBS Only

PLCB3 Monoclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 66668-1-PBS
Clone No.1B8B3

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

Phospholipase C-beta-3, Phospholipase C beta 3, Phosphoinositide phospholipase C-beta-3, EC:3.1.4.11, 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-3

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66668-1-PBS targets PLCB3 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen PLCB3 fusion protein Ag15845 相同性解析による交差性が予測される生物種
Full Name phospholipase C, beta 3 (phosphatidylinositol-specific)
Calculated molecular weight 1234 aa, 139 kDa
Observed molecular weight 150 kDa
GenBank accession numberBC142681
Gene Symbol PLCB3
Gene ID (NCBI) 5331
RRIDAB_2882022
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ01970
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

PLCB3 is a member of the phosphoinositide phospholipase C beta enzyme family that catalyze the production of the secondary messengers diacylglycerol and inositol 1,4,5-triphosphate from phosphatidylinositol in G-protein-linked receptor-mediated signal transduction. Six subfamilies of PLCs (B, G, D, E, Z and H) constitute part of ubiquitous signaling cascades that translate hormonal signals into intracellular events, leading to alternations in cell function. PLCB isoforms 1-4 are stimulated by G-protein activation (Gαq/11 and/or Gβγ). Independent of its enzymatic activity, PLCB3 inhibits the proliferation of hematopoietic stem cells (HSCs) and myeloid cells.