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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PLCD1 Monoclonal antibody

PLCD1 Monoclonal Antibody for WB, IHC, FC (Intra), ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

1D10B11

Cat no : 67064-1-Ig

Synonyms

Phospholipase C III, Phospholipase C delta 1, Phosphoinositide phospholipase C-delta-1, EC:3.1.4.11, 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase delta-1

Validation Data Gallery

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  • Western Blot (WB) analysis of human testis tissue using PLCD1 Monoclonal antibody (67064-1-Ig)

    WB analysis of human testis using 67064-1-Ig

    human testis tissue were subjected to SDS PAGE followed by western blot with 67064-1-Ig (PLCD1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of K-562 cells using PLCD1 Monoclonal antibody (67064-1-Ig)

    WB analysis of K-562 using 67064-1-Ig

    K-562 cells were subjected to SDS PAGE followed by western blot with 67064-1-Ig (PLCD1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human testis tissue using PLCD1 Monoclonal antibody (67064-1-Ig)

    IHC staining of human testis using 67064-1-Ig

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 67064-1-Ig (PLCD1 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human testis tissue using PLCD1 Monoclonal antibody (67064-1-Ig)

    IHC staining of human testis using 67064-1-Ig

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 67064-1-Ig (PLCD1 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Flow cytometry (FC) experiment of K-562 cells using PLCD1 Monoclonal antibody (67064-1-Ig)

    FC experiment of K-562 using 67064-1-Ig

    1x10^6 K-562 cells were intracellularly stained with 0.2 ug PLCD1 Monoclonal antibody (67064-1-Ig, Clone:1D10B11) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.2 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive WB detected inhuman testis tissue, K-562 cells
Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC (Intra) detected inK-562 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:150-1:600
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67064-1-Ig targets PLCD1 in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen PLCD1 fusion protein Ag5567 相同性解析による交差性が予測される生物種
Full Name phospholipase C, delta 1
Calculated molecular weight 86 kDa
Observed molecular weight 85 kDa
GenBank accession numberBC050382
Gene symbol PLCD1
Gene ID (NCBI) 5333
RRIDAB_2882374
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for PLCD1 antibody 67064-1-IgDownload protocol
IHC protocol for PLCD1 antibody 67064-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols