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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PLCD3 Polyclonal antibody

PLCD3 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 16792-1-AP

Synonyms

Phospholipase C-delta-3, phospholipase C, delta 3, Phospholipase C delta 3, Phosphoinositide phospholipase C-delta-3, EC:3.1.4.11

Validation Data Gallery

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  • Western Blot (WB) analysis of SH-SY5Y cells using PLCD3 Polyclonal antibody (16792-1-AP)

    WB analysis of SH-SY5Y using 16792-1-AP

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 16792-1-AP (PLCD3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A549 cells using PLCD3 Polyclonal antibody (16792-1-AP)

    WB analysis of A549 using 16792-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 16792-1-AP (PLCD3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of U2OS cells using PLCD3 Polyclonal antibody (16792-1-AP)

    WB analysis of U2OS using 16792-1-AP

    U2OS cells were subjected to SDS PAGE followed by western blot with 16792-1-AP (PLCD3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human intrahepatic cholangiocarcinoma tissue using PLCD3 Polyclonal antibody (16792-1-AP)

    IHC staining of human intrahepatic cholangiocarcinoma using 16792-1-AP

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 16792-1-AP (PLCD3 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human intrahepatic cholangiocarcinoma tissue using PLCD3 Polyclonal antibody (16792-1-AP)

    IHC staining of human intrahepatic cholangiocarcinoma using 16792-1-AP

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 16792-1-AP (PLCD3 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inSH-SY5Y cells, A549 cells, U2OS cells
Positive IHC detected inhuman intrahepatic cholangiocarcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

16792-1-AP targets PLCD3 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PLCD3 fusion protein Ag10249 相同性解析による交差性が予測される生物種
Full Name phospholipase C, delta 3
Calculated molecular weight 789 aa, 89 kDa
Observed molecular weight 85 kDa
GenBank accession numberBC072384
Gene symbol PLCD3
Gene ID (NCBI) 113026
RRIDAB_2878316
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Phospholipase C Delta 3 (PLCD3) is a protein-coding gene located on chromosome 17 and a member of the phospholipase C family. PLCD3 is expressed broadly across different tissues, with high expression levels observed in the colon and urinary bladder, among others. The enzyme localizes to the plasma membrane and requires calcium for activation. Spermine, sphingosine, and several phospholipids can inhibit its activity. Recent studies have implicated PLCD3 in various types of cancer. In summary, PLCD3 is an important enzyme involved in cellular signaling and has been implicated in the development and progression of various cancers, making it a potential target for therapeutic interventions.

Protocols

Product Specific Protocols
WB protocol for PLCD3 antibody 16792-1-APDownload protocol
IHC protocol for PLCD3 antibody 16792-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
pigWB

J Cell Biochem

Phospholipase C inhibits apoptosis of porcine oocytes cultured in vitro.

Authors - Hua Li Chen
View Article
humanWB

Oncol Rep

PLCD3, a flotillin2-interacting protein, is involved in proliferation, migration and invasion of nasopharyngeal carcinoma cells.

Authors - Weidong Liu
View Article