Validation Data Gallery
Tested Applications
| Positive WB detected in | SH-SY5Y cells, A549 cells, U2OS cells |
| Positive IHC detected in | human intrahepatic cholangiocarcinoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 2 publications below |
Product Information
16792-1-AP targets PLCD3 in WB, IHC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, pig |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | PLCD3 fusion protein Ag10249 相同性解析による交差性が予測される生物種 |
| Full Name | phospholipase C, delta 3 |
| Calculated molecular weight | 789 aa, 89 kDa |
| Observed molecular weight | 85 kDa |
| GenBank accession number | BC072384 |
| Gene Symbol | PLCD3 |
| Gene ID (NCBI) | 113026 |
| RRID | AB_2878316 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8N3E9 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Phospholipase C Delta 3 (PLCD3) is a protein-coding gene located on chromosome 17 and a member of the phospholipase C family. PLCD3 is expressed broadly across different tissues, with high expression levels observed in the colon and urinary bladder, among others. The enzyme localizes to the plasma membrane and requires calcium for activation. Spermine, sphingosine, and several phospholipids can inhibit its activity. Recent studies have implicated PLCD3 in various types of cancer. In summary, PLCD3 is an important enzyme involved in cellular signaling and has been implicated in the development and progression of various cancers, making it a potential target for therapeutic interventions.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for PLCD3 antibody 16792-1-AP | Download protocol |
| IHC protocol for PLCD3 antibody 16792-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Oncol Rep PLCD3, a flotillin2-interacting protein, is involved in proliferation, migration and invasion of nasopharyngeal carcinoma cells. |