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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PLCD4 Polyclonal antibody

PLCD4 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 10589-2-AP

Synonyms

hPLCD4, Phospholipase C delta 4, phospholipase C, delta 4, PLC delta 4, PLCD4

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse skeletal muscle tissue using PLCD4 Polyclonal antibody (10589-2-AP)

    WB analysis of mouse skeletal muscle using 10589-2-AP

    mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 10589-2-AP (PLCD4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using PLCD4 Polyclonal antibody (10589-2-AP)

    WB analysis using 10589-2-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10589-2-AP (PLCD4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using PLCD4 Polyclonal antibody (10589-2-AP)

    IHC staining of human pancreas cancer using 10589-2-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 10589-2-AP (PLCD4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using PLCD4 Polyclonal antibody (10589-2-AP)

    IHC staining of human pancreas cancer using 10589-2-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 10589-2-AP (PLCD4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inmouse skeletal muscle tissue, rat skeletal muscle tissue
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10589-2-AP targets PLCD4 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PLCD4 fusion protein Ag0937 相同性解析による交差性が予測される生物種
Full Name phospholipase C, delta 4
Calculated molecular weight 85-90 kDa
Observed molecular weight100-110 kDa
GenBank accession numberBC006355
Gene symbol PLCD4
Gene ID (NCBI) 84812
RRIDAB_2163398
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PLCD4 is a member of the delta class of phospholipase C enzymes. Phospholipase C enzymes play a critical role in many cellular processes by hydrolyzing phosphatidylinositol 4,5-bisphosphate into two intracellular second messengers, inositol 1,4,5-trisphosphate and diacylglycerol. Expression of this gene may be a marker for cancer.

Protocols

Product Specific Protocols
WB protocol for PLCD4 antibody 10589-2-APDownload protocol
IHC protocol for PLCD4 antibody 10589-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols