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  • Featured Product
  • KD/KO Validated

PLCD4 Polyclonal antibody, PBS Only

PLCD4 Polyclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 30133-1-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using PLCD4 Polyclonal antibody (30133-1-AP)

    WB analysis of HeLa using 30133-1-AP (same clone as 30133-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 30133-1-AP (PLCD4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30133-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using PLCD4 Polyclonal antibody (30133-1-AP)

    WB analysis of HeLa using 30133-1-AP (same clone as 30133-1-PBS)

    WB result of PLCD4 antibody (30133-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PLCD4 transfected HeLa cells. This data was developed using the same antibody clone with 30133-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat testis tissue using PLCD4 Polyclonal antibody (30133-1-AP)

    WB analysis of rat testis using 30133-1-AP (same clone as 30133-1-PBS)

    rat testis tissue were subjected to SDS PAGE followed by western blot with 30133-1-AP (PLCD4 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30133-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using PLCD4 Polyclonal antibody (30133-1-AP)

    WB analysis using 30133-1-AP (same clone as 30133-1-PBS)

    human testis tissue were subjected to SDS PAGE followed by western blot with 30133-1-AP (PLCD4 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30133-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using PLCD4 Polyclonal antibody (30133-1-AP)

    IHC staining of mouse testis using 30133-1-AP (same clone as 30133-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 30133-1-AP (PLCD4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30133-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using PLCD4 Polyclonal antibody (30133-1-AP)

    IHC staining of human pancreas cancer using 30133-1-AP (same clone as 30133-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 30133-1-AP (PLCD4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30133-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

30133-1-PBS targets PLCD4 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PLCD4 fusion protein Ag32651 相同性解析による交差性が予測される生物種
Full Name phospholipase C, delta 4
Calculated molecular weight 85-90 kDa
Observed molecular weight90 kDa
GenBank accession numberBC006355
Gene symbol PLCD4
Gene ID (NCBI) 84812
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

PLCD4(1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase delta-4), also known as hPLCD4. It is highly expressed in skeletal muscle and kidney tissues. It may be an important enzyme for intracellular Ca²⁺ mobilization in the zona pellucida-induced acrosome reaction. PLCD4 cooperates with nuclear PKC to regulate liver regeneration. Overexpression of PLCD4 upregulates Erk signaling pathway and proliferation (PMID: 15140260).