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Validation Data Gallery

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  • Western Blot (WB) analysis of Ramos cells using PLCG2 Monoclonal antibody (67011-1-Ig)

    WB analysis of Ramos using 67011-1-Ig (same clone as 67011-1-PBS)

    Ramos cells were subjected to SDS PAGE followed by western blot with 67011-1-Ig (PLCG2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67011-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Karpas-422 cells using PLCG2 Monoclonal antibody (67011-1-Ig)

    WB analysis of Karpas-422 using 67011-1-Ig (same clone as 67011-1-PBS)

    Karpas-422 cells were subjected to SDS PAGE followed by western blot with 67011-1-Ig (PLCG2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67011-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rabbit spleen tissue using PLCG2 Monoclonal antibody (67011-1-Ig)

    WB analysis of rabbit spleen using 67011-1-Ig (same clone as 67011-1-PBS)

    Rabbit spleen tissue was subjected to SDS PAGE followed by western blot with 67011-1-Ig (PLCG2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67011-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using PLCG2 Monoclonal antibody (67011-1-Ig)

    WB analysis using 67011-1-Ig (same clone as 67011-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67011-1-Ig (PLCG2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67011-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using PLCG2 Monoclonal antibody (67011-1-Ig)

    IHC staining of human tonsillitis using 67011-1-Ig (same clone as 67011-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67011-1-Ig (PLCG2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67011-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using PLCG2 Monoclonal antibody (67011-1-Ig)

    IHC staining of human tonsillitis using 67011-1-Ig (same clone as 67011-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67011-1-Ig (PLCG2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67011-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

PLCG2 Monoclonal antibody, PBS Only

PLCG2 Monoclonal Antibody for WB, IHC, ELISA
Cat No. 67011-1-PBS
Clone No.2F8H5

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

Phospholipase C IV, Phospholipase C gamma 2, Phosphoinositide phospholipase C-gamma-2, EC:3.1.4.11, 2F8H5

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67011-1-PBS targets PLCG2 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen PLCG2 fusion protein Ag24355 相同性解析による交差性が予測される生物種
Full Name phospholipase C, gamma 2 (phosphatidylinositol-specific)
Calculated molecular weight 148 kDa
Observed molecular weight 148 kDa
GenBank accession numberBC007565
Gene Symbol PLCG2
Gene ID (NCBI) 5336
RRIDAB_2882328
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP16885
Storage Buffer PBS only
Storage ConditionsStore at -80°C.

Background Information

Phospholipase C-gamma2 (PLCG2) is a transmembrane signaling enzyme that catalyzes the conversion of 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate to 1D-myo-inositol 1,4,5-trisphosphate (IP3) and diacylglycerol (DAG) using calcium as a cofactor.IP3 and DAG are second messenger molecules important for transmitting signals from growth factor receptors and immune system receptors across the cell membrane. Mutations in this gene have been found in autoinflammation, antibody deficiency, and immune dysregulation syndrome and familial cold autoinflammatory syndrome 3.