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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PLD1 Polyclonal antibody

PLD1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 18355-1-AP

Synonyms

PLD 1, Phospholipase D1, Phosphatidylcholine-hydrolyzing phospholipase D1, hPLD1, EC:3.1.4.4

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PLD1 Polyclonal antibody (18355-1-AP)

    WB analysis using 18355-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 18355-1-AP (PLD1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse heart tissue using PLD1 Polyclonal antibody (18355-1-AP)

    IHC staining of mouse heart using 18355-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 18355-1-AP (PLD1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using PLD1 Polyclonal antibody (18355-1-AP)

    IF Staining of HeLa using 18355-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using PLD1 antibody (18355-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inHeLa cells
Positive IHC detected inmouse heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

18355-1-AP targets PLD1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PLD1 fusion protein Ag13227 相同性解析による交差性が予測される生物種
Full Name phospholipase D1, phosphatidylcholine-specific
Calculated molecular weight 1074 aa, 124 kDa
Observed molecular weight112-124 kDa
GenBank accession numberBC068976
Gene symbol PLD1
Gene ID (NCBI) 5337
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PLD1(Phospholipase D1) belongs to the phospholipase D family. It is implicated in the release process of a variety of hormones, most likely at a late stage of exocytosis through the production of lipids that are essential for the fusion process and seem to be also essential for fast neurotransmitter release. PLD1 is primarily localized in vesicular structures such as endosomes, lysosomes, and autophagosomes.

Protocols

Product Specific Protocols
WB protocol for PLD1 antibody 18355-1-APDownload protocol
IHC protocol for PLD1 antibody 18355-1-APDownload protocol
IF protocol for PLD1 antibody 18355-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IF

J Cell Biol

Class III PI-3-kinase activates phospholipase D in an amino acid-sensing mTORC1 pathway.

Authors - Yoon Mee-Sup MS
View Article