Validation Data Gallery
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Tested Applications
| Positive WB detected in | HeLa cells |
| Positive IHC detected in | mouse heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
| IF | See 1 publications below |
Product Information
18355-1-AP targets PLD1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | PLD1 fusion protein Ag13227 相同性解析による交差性が予測される生物種 |
| Full Name | phospholipase D1, phosphatidylcholine-specific |
| Calculated molecular weight | 1074 aa, 124 kDa |
| Observed molecular weight | 112-124 kDa |
| GenBank accession number | BC068976 |
| Gene Symbol | PLD1 |
| Gene ID (NCBI) | 5337 |
| RRID | AB_2163859 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q13393 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
PLD1(Phospholipase D1) belongs to the phospholipase D family. It is implicated in the release process of a variety of hormones, most likely at a late stage of exocytosis through the production of lipids that are essential for the fusion process and seem to be also essential for fast neurotransmitter release. PLD1 is primarily localized in vesicular structures such as endosomes, lysosomes, and autophagosomes.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for PLD1 antibody 18355-1-AP | Download protocol |
| IHC protocol for PLD1 antibody 18355-1-AP | Download protocol |
| IF protocol for PLD1 antibody 18355-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Cell Biol Class III PI-3-kinase activates phospholipase D in an amino acid-sensing mTORC1 pathway. |