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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PLDN Monoclonal antibody (66558-1-Ig)

    WB analysis using 66558-1-Ig (same clone as 66558-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66558-1-Ig (PLDN antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66558-1-PBS in a different storage buffer formulation.

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PLDN Monoclonal antibody, PBS Only

PLDN Monoclonal Antibody for WB, Indirect ELISA
Cat No. 66558-1-PBS
Clone No.4F4C11

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

WB, Indirect ELISA

Synonyms

PA, PALLID, Pallid protein homolog, Pallidin, pallidin homolog (mouse), PLDN

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66558-1-PBS targets PLDN in WB, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen PLDN fusion protein Ag18095 相同性解析による交差性が予測される生物種
Full Name pallidin homolog (mouse)
Calculated molecular weight 23~24 kDa
Observed molecular weight 26 kDa
GenBank accession numberBC004819
Gene Symbol PLDN
Gene ID (NCBI) 26258
RRIDAB_2881919
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9UL45
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Pallidin, also named as PLDN and PA, is involved in the development of lysosome-related organelles, such as melanosomes and platelet-dense granules. It may play a role in intracellular vesicle trafficking, particularly in the vesicle-docking and fusion process. Pallidin interacts with syntaxin-12 which is a t-SNARE protein that mediates vesicle docking and fusion.