PLEC Polyclonal antibody
PLEC Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
WB, IHC, IF/ICC, ELISA
Conjugate
Unconjugated
Cat no : 29170-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HEK-293 cells, mouse brain tissue, mouse kidney tissue |
| Positive IHC detected in | mouse heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | A549 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:16000 |
| Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
29170-1-AP targets PLEC in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | PLEC fusion protein Ag29418 相同性解析による交差性が予測される生物種 |
| Full Name | plectin 1, intermediate filament binding protein 500kDa |
| Calculated molecular weight | 532 kDa |
| Observed molecular weight | 510 kDa |
| GenBank accession number | NM_201380 |
| Gene symbol | Plectin |
| Gene ID (NCBI) | 5339 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Plectin is a large (≥500-kDa) protein that is normally expressed in various tissues including skin, muscle, and brain. It binds to a number of cytoskeletal proteins including microtubules and intermediate filaments and is involved in establishment and dynamic modulation of the cytoskeletal network. In normal cells, it plays a crucial role in cytoskeleton network organization. In keratinocytes, plectin is concentrated at the basal surface, where it links intermediate filaments to the cytoplasmic domain of transmembrane glycoproteins such as integrin beta-4. (PMID: 24218614, PMID: 28281696, PMID: 23750011)
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for PLEC antibody 29170-1-AP | Download protocol |
| IHC protocol for PLEC antibody 29170-1-AP | Download protocol |
| IF protocol for PLEC antibody 29170-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Marina (Verified Customer) (03-22-2024) | Samples are two tumour (glioblastoma) cell lines (TC1, TC2). Gradient gel 4-15 %. Dilution 1:2000, primary antibody incubation 4ºC overnight, secondary antibody incubation 1 h room temperature. The blot was incubated with anti-plectin antibody after incubation with anti-spectrin beta chain (ref. 67978-1), stripping and re-blocking. The bands appear at the expected size of the target protein plectin (exposure 2 minutes).
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