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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PLEC Recombinant antibody

PLEC Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

240891A10

Cat no : 83898-1-RR

Synonyms

PLEC1, Plectin, PLTN, Plectin 1, PLEC1b

Validation Data Gallery

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  • Western Blot (WB) analysis of A431 cells using PLEC Recombinant antibody (83898-1-RR)

    WB analysis of A431 using 83898-1-RR

    A431 cells were subjected to SDS PAGE followed by western blot with 83898-1-RR (PLEC antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using PLEC Recombinant antibody (83898-1-RR)

    WB analysis using 83898-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83898-1-RR (PLEC antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 83898-1-RR

    Affinity and Kinetic Characterization of 83898-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83898-1-RR against Human PLEC were performed. The affinity constant is 52.7 pM.

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Tested Applications

Positive WB detected inHaCaT cells, A431 cells, HEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83898-1-RR is PLEC Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen PLEC fusion protein Ag29418 相同性解析による交差性が予測される生物種
Full Name plectin 1, intermediate filament binding protein 500kDa
Calculated molecular weight 532 kDa
Observed molecular weight 510 kDa
GenBank accession numberNM_201380
Gene symbol Plectin
Gene ID (NCBI) 5339
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Plectin is a large (≥500-kDa) protein that is normally expressed in various tissues including skin, muscle, and brain. It binds to a number of cytoskeletal proteins including microtubules and intermediate filaments and is involved in establishment and dynamic modulation of the cytoskeletal network. In normal cells, it plays a crucial role in cytoskeleton network organization. In keratinocytes, plectin is concentrated at the basal surface, where it links intermediate filaments to the cytoplasmic domain of transmembrane glycoproteins such as integrin beta-4. (PMID: 24218614, PMID: 28281696, PMID: 23750011)

Protocols

Product Specific Protocols
WB protocol for PLEC antibody 83898-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols