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PLEKHA1 Polyclonal antibody

PLEKHA1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 26830-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, ELISA

TAPP-1, TAPP1, TAPP 1, Tandem PH domain-containing protein 1, Pleckstrin homology domain-containing family A member 1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -


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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換/補填対応を承ります。詳細はこちらをご覧ください。


Tested Applications

Positive WB detected inHeLa cells, A549 cells, Jurkat cells, MOLT-4 cells
Positive IP detected inA549 cells
Positive IHC detected inhuman colon tissue, human pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

26830-1-AP targets PLEKHA1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PLEKHA1 fusion protein Ag25285 相同性解析による交差性が予測される生物種
Full Name pleckstrin homology domain containing, family A (phosphoinositide binding specific) member 1
Calculated molecular weight 46 kDa
Observed molecular weight 46 kDa
GenBank accession numberBC001136
Gene Symbol PLEKHA1
Gene ID (NCBI) 59338
RRIDAB_2880651
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9HB21
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Pleckstrin homology (PH) domain is commonly found in eukaryotic signaling proteins and possesses multiple functions including the abilities to bind inositol phosphates and various proteins. The tandem PH domain containing protein-1 (TAPP1) or PH domain containing-family A (phosphoinositide binding specific) member 1 (PLEKHA1), interacts strongly and specifically with phosphatidylinositol 3,4-trisphosphate [PtdIns(3,4)P(2)], which is one of the immediate breakdown products of PtdIns(3,4,5) P (3) and functions as a signalling molecule in insulin- and growth-factor-stimulated pathways. TAPP1 is also associated with the protein- tyrosine-phosphatase-like protein-1 (PTPL1 also known as FAP-1) and maintains PTPL1 in cytoplasm. By binding to PtdIns(3,4) P (2) and PTPL1, TAPP1 may regulate the membrane localization of PTPL1."

Protocols

Product Specific Protocols
WB protocol for PLEKHA1 antibody 26830-1-APDownload protocol
IHC protocol for PLEKHA1 antibody 26830-1-APDownload protocol
IF protocol for PLEKHA1 antibody 26830-1-APDownload protocol
IP protocol for PLEKHA1 antibody 26830-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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