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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PLEKHA1 Polyclonal antibody

PLEKHA1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 26830-1-AP

Synonyms

TAPP-1, TAPP1, TAPP 1, Tandem PH domain-containing protein 1, Pleckstrin homology domain-containing family A member 1

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using PLEKHA1 Polyclonal antibody (26830-1-AP)

    WB analysis of HeLa using 26830-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 26830-1-AP (PLEKHA1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using PLEKHA1 Polyclonal antibody (26830-1-AP)

    WB analysis using 26830-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 26830-1-AP (PLEKHA1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A549 cells using PLEKHA1 Polyclonal antibody (26830-1-AP)

    WB analysis of A549 using 26830-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 26830-1-AP (PLEKHA1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of A549 cells using PLEKHA1 Polyclonal antibody (26830-1-AP)

    IP experiment of A549 using 26830-1-AP

    IP result of anti-PLEKHA1 (IP:26830-1-AP, 4ug; Detection:26830-1-AP 1:1000) with A549 cells lysate 1600 ug.

  • Immunohistochemistry (IHC) staining of human colon tissue using PLEKHA1 Polyclonal antibody (26830-1-AP)

    IHC staining of human colon using 26830-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 26830-1-AP (PLEKHA1 antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human colon tissue using PLEKHA1 Polyclonal antibody (26830-1-AP)

    IHC staining of human colon using 26830-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 26830-1-AP (PLEKHA1 antibody) at dilution of 1:200 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human pancreas tissue using PLEKHA1 Polyclonal antibody (26830-1-AP)

    IHC staining of human pancreas using 26830-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 26830-1-AP (PLEKHA1 antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human pancreas tissue using PLEKHA1 Polyclonal antibody (26830-1-AP)

    IHC staining of human pancreas using 26830-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 26830-1-AP (PLEKHA1 antibody) at dilution of 1:200 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using PLEKHA1 Polyclonal antibody (26830-1-AP)

    IF Staining of A549 using 26830-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using PLEKHA1 antibody (26830-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Tested Applications

Positive WB detected inHeLa cells, A549 cells, Jurkat cells, MOLT-4 cells
Positive IP detected inA549 cells
Positive IHC detected inhuman colon tissue, human pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

26830-1-AP targets PLEKHA1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PLEKHA1 fusion protein Ag25285 相同性解析による交差性が予測される生物種
Full Name pleckstrin homology domain containing, family A (phosphoinositide binding specific) member 1
Calculated molecular weight 46 kDa
Observed molecular weight 46 kDa
GenBank accession numberBC001136
Gene symbol PLEKHA1
Gene ID (NCBI) 59338
RRIDAB_2880651
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Pleckstrin homology (PH) domain is commonly found in eukaryotic signaling proteins and possesses multiple functions including the abilities to bind inositol phosphates and various proteins. The tandem PH domain containing protein-1 (TAPP1) or PH domain containing-family A (phosphoinositide binding specific) member 1 (PLEKHA1), interacts strongly and specifically with phosphatidylinositol 3,4-trisphosphate [PtdIns(3,4)P(2)], which is one of the immediate breakdown products of PtdIns(3,4,5) P (3) and functions as a signalling molecule in insulin- and growth-factor-stimulated pathways. TAPP1 is also associated with the protein- tyrosine-phosphatase-like protein-1 (PTPL1 also known as FAP-1) and maintains PTPL1 in cytoplasm. By binding to PtdIns(3,4) P (2) and PTPL1, TAPP1 may regulate the membrane localization of PTPL1."

Protocols

Product Specific Protocols
WB protocol for PLEKHA1 antibody 26830-1-APDownload protocol
IHC protocol for PLEKHA1 antibody 26830-1-APDownload protocol
IF protocol for PLEKHA1 antibody 26830-1-APDownload protocol
IP protocol for PLEKHA1 antibody 26830-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols