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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PLEKHA2 Polyclonal antibody (32169-1-AP)

    WB analysis using 32169-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 32169-1-AP (PLEKHA2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using PLEKHA2 Polyclonal antibody (32169-1-AP)

    IF Staining of A431 using 32169-1-AP

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using PLEKHA2 antibody (32169-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using PLEKHA2 Polyclonal antibody (32169-1-AP)

    IF Staining of HeLa using 32169-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PLEKHA2 antibody (32169-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

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PLEKHA2 Polyclonal antibody

PLEKHA2 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 32169-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

TAPP-2, TAPP2, Tandem PH domain-containing protein 2, Pleckstrin homology domain-containing family A member 2, Pleckstrin Homology Domain Containing A2

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, JurKat cells, RT-4 cells
Positive IF/ICC detected inHeLa cells, A431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

32169-1-AP targets PLEKHA2 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PLEKHA2 fusion protein Ag36596 相同性解析による交差性が予測される生物種
Full Name pleckstrin homology domain containing, family A (phosphoinositide binding specific) member 2
Observed molecular weight42 kDa
GenBank accession numberBC166639
Gene Symbol PLEKHA2
Gene ID (NCBI) 59339
RRIDAB_3670213
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
UNIPROT IDQ9HB19
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PLEKHA2 (Pleckstrin homology domain containing A2), also known as TAPP2, is a member of the PLEKHA family of proteins.PLEKHA2 is a junctional protein with a Pleckstrin homology domain that is involved in signaling after lymphocyte activation and plays an important role in cytoskeletal reorganization driven by phosphatidylinositol 3-kinase.

Protocols

Product Specific Protocols
WB protocol for PLEKHA2 antibody 32169-1-APDownload protocol
IF protocol for PLEKHA2 antibody 32169-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols