Validation Data Gallery

Filter: AllWBIHCIF/ICC

WB analysis of human skeletal muscle using 20389-1-AP
human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 20389-1-AP (PLEKHF1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of human kidney using 20389-1-AP
human kidney tissue were subjected to SDS PAGE followed by western blot with 20389-1-AP (PLEKHF1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse lung using 20389-1-AP
mouse lung tissue were subjected to SDS PAGE followed by western blot with 20389-1-AP (PLEKHF1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse thymus using 20389-1-AP
mouse thymus tissue were subjected to SDS PAGE followed by western blot with 20389-1-AP (PLEKHF1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse skeletal muscle using 20389-1-AP
mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 20389-1-AP (PLEKHF1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse spleen using 20389-1-AP
mouse spleen tissue were subjected to SDS PAGE followed by western blot with 20389-1-AP (PLEKHF1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IHC staining of human brain using 20389-1-AP
Immunohistochemical analysis of paraffin-embedded human brain slide using 20389-1-AP (PLEKHF1 Antibody) at dilution of 1:50.
IHC staining of human brain using 20389-1-AP
Immunohistochemical analysis of paraffin-embedded human brain slide using 20389-1-AP (PLEKHF1 Antibody) at dilution of 1:50.
IF Staining of HepG2 using 20389-1-AP
Immunofluorescent analysis of HepG2 cells, using PLEKHF1 antibody 20389-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

Featured Product
KD/KO Validated

PLEKHF1 Polyclonal antibody

PLEKHF1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 20389-1-AP

Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

Apoptosis inducing protein, APPD, LAPF, Phafin 1, PHAFIN1, PLEKHF1, ZFYVE15

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Overview

Tested Applications
Product Information
Publications (2)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	human skeletal muscle tissue, human kidney tissue, mouse lung tissue, mouse skeletal muscle tissue, mouse spleen tissue, mouse thymus tissue
Positive IHC detected in	human brain tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunohistochemistry (IHC)	IHC : 1:20-1:200
Immunofluorescence (IF)/ICC	IF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 2 publications below
WB	See 2 publications below
IF	See 1 publications below

Product Information

20389-1-AP targets PLEKHF1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	PLEKHF1 fusion protein Ag14228 相同性解析による交差性が予測される生物種
Full Name	pleckstrin homology domain containing, family F (with FYVE domain) member 1
Calculated molecular weight	279 aa, 31 kDa
Observed molecular weight	31 kDa
GenBank accession number	BC002744
Gene Symbol	PLEKHF1
Gene ID (NCBI)	79156
RRID	AB_10666430
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q96S99
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

PLEKHF1, also named as APPD, LAPF, and ZFYVE15, is a 279 amino acid protein, which contains one PH domain and one FYVE-type zinc finger. PLEKHF1 translocates to lysosome during apoptosis, localizing in the nucleus and cytoplsam. PLEKHF1 is highly expressed in heart and skeletal muscle and weakly expressed in brain, thymus, spleen, kidney, liver, small intestine, placenta and lung. PLEKHF1 may induce apoptosis through the lysosomal-mitochondrial pathway. PLEKHF1 modulates the protein content of the plasma membrane and was involved in different endocytosis events.

Protocols

Product Specific Protocols
WB protocol for PLEKHF1 antibody 20389-1-AP	Download protocol
IHC protocol for PLEKHF1 antibody 20389-1-AP	Download protocol
IF protocol for PLEKHF1 antibody 20389-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Breast Cancer Res Functional characterization of the 19q12 amplicon in grade III breast cancers. Authors - Natrajan Rachael R KD Validated View Article
mouse	WB,IF	Sci China Life Sci Local administration of liposomal-based Plekhf1 gene therapy attenuates pulmonary fibrosis by modulating macrophage polarization Authors - Lifeng Yan KD Validated View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of human skeletal muscle using 20389-1-AP

WB analysis of human kidney using 20389-1-AP

WB analysis of mouse lung using 20389-1-AP

WB analysis of mouse thymus using 20389-1-AP

WB analysis of mouse skeletal muscle using 20389-1-AP

WB analysis of mouse spleen using 20389-1-AP

IHC staining of human brain using 20389-1-AP