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Validation Data Gallery

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  • Western Blot (WB) analysis of LNCaP cells using PLEKHH3 Polyclonal antibody (28154-1-AP)

    WB analysis of LNCaP using 28154-1-AP

    LNCaP cells were subjected to SDS PAGE followed by western blot with 28154-1-AP (PLEKHH3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using PLEKHH3 Polyclonal antibody (28154-1-AP)

    WB analysis of mouse brain using 28154-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 28154-1-AP (PLEKHH3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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PLEKHH3 Polyclonal antibody

PLEKHH3 Polyclonal Antibody for WB, ELISA
Cat No. 28154-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse

Applications

WB, ELISA

Synonyms

FLJ21019, PLEKHH3

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inLNCaP cells, mouse brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

28154-1-AP targets PLEKHH3 in WB, ELISA applications and shows reactivity with Human, Mouse samples.

Tested Reactivity Human, Mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PLEKHH3 fusion protein Ag28001 相同性解析による交差性が予測される生物種
Full Name pleckstrin homology domain containing, family H (with MyTH4 domain) member 3
Calculated molecular weight 793 aa, 85 kDa
Observed molecular weight 85 and 80 kDa
GenBank accession numberBC052978
Gene Symbol PLEKHH3
Gene ID (NCBI) 79990
RRIDAB_2881076
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ7Z736
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PLEKHH3, a gene related to endothelial tight junction, is associated with obstructive sleep apnea (OSA) and angiogenesis. It has been reported that the expression of PLEKHH3 protein was increased in the OSA patients. PLEKHH3 may play a key role in OSA and angiogenesis by specifically regulating the endothelial cells formation of tight junctions. 28154-1-AP detects the isoforms of PLEKHH3 protein around 80-85 kDa in SDS-PAGE.(PMID:28520763)

Protocols

Product Specific Protocols
WB protocol for PLEKHH3 antibody 28154-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols