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Target

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Species Reactivity

Species Reactivity

Conjugate

Conjugate

PLEKHM1 Polyclonal antibody

PLEKHM1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 16202-1-AP

Synonyms

162 kDa adapter protein, AP162, B2, KIAA0356, OPTB6, PLEKHM1

Validation Data Gallery

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  • Western Blot (WB) analysis of ROS1728 cells using PLEKHM1 Polyclonal antibody (16202-1-AP)

    WB analysis of ROS1728 using 16202-1-AP

    ROS1728 cells were subjected to SDS PAGE followed by western blot with 16202-1-AP (PLEKHM1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse liver tissue using PLEKHM1 Polyclonal antibody (16202-1-AP)

    WB analysis of mouse liver using 16202-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 16202-1-AP (PLEKHM1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human kidney tissue using PLEKHM1 Polyclonal antibody (16202-1-AP)

    IHC staining of human kidney using 16202-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 16202-1-AP (PLEKHM1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human kidney tissue using PLEKHM1 Polyclonal antibody (16202-1-AP)

    IHC staining of human kidney using 16202-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 16202-1-AP (PLEKHM1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using PLEKHM1 Polyclonal antibody (16202-1-AP)

    IHC staining of human breast cancer using 16202-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 16202-1-AP (PLEKHM1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using PLEKHM1 Polyclonal antibody (16202-1-AP)

    IHC staining of human breast cancer using 16202-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 16202-1-AP (PLEKHM1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using PLEKHM1 Polyclonal antibody (16202-1-AP)

    IHC staining of human liver cancer using 16202-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16202-1-AP (PLEKHM1 antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using PLEKHM1 Polyclonal antibody (16202-1-AP)

    IHC staining of human liver cancer using 16202-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16202-1-AP (PLEKHM1 antibody) at dilution of 1:200 (under 40x lens).

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Tested Applications

Positive WB detected inROS1728 cells, mouse liver tissue
Positive IHC detected inhuman kidney tissue, human liver cancer tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

16202-1-AP targets PLEKHM1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PLEKHM1 fusion protein Ag9145 相同性解析による交差性が予測される生物種
Full Name pleckstrin homology domain containing, family M (with RUN domain) member 1
Calculated molecular weight 117 kDa
Observed molecular weight 117 kDa
GenBank accession numberBC064361
Gene symbol PLEKHM1
Gene ID (NCBI) 9842
RRIDAB_2878228
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for PLEKHM1 antibody 16202-1-APDownload protocol
IHC protocol for PLEKHM1 antibody 16202-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Autophagy

SDC1-dependent TGM2 determines radiosensitivity in glioblastoma by coordinating EPG5-mediated fusion of autophagosomes with lysosomes

Authors - Wang Zheng
View Article
mouseWB

Nutrients

Improving Lipophagy by Restoring Rab7 Cycle: Protective Effects of Quercetin on Ethanol-Induced Liver Steatosis.

Authors - Hongkun Lin
View Article