Perilipin 1 Polyclonal antibody
Perilipin 1 Polyclonal Antibody for WB, IHC, IF-P, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
WB, IHC, IF-P, IP, ELISA
Conjugate
Unconjugated
Cat no : 27716-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | 3T3-L1 cells, MCF-7 cells, NIH/3T3 cells, mouse liver tissue |
| Positive IP detected in | NIH/3T3 cells |
| Positive IHC detected in | mouse brown adipose tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse brown adipose tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 2 publications below |
| IHC | See 1 publications below |
Product Information
27716-1-AP targets Perilipin 1 in WB, IHC, IF-P, IP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Perilipin 1 fusion protein Ag26426 相同性解析による交差性が予測される生物種 |
| Full Name | perilipin |
| Calculated molecular weight | 56 kDa |
| Observed molecular weight | 62-67 kDa |
| GenBank accession number | NM_001145311 |
| Gene symbol | Perilipin 1 |
| Gene ID (NCBI) | 5346 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
PLIN1, also known as Perilipin 1, PLIN, PERI, belongs to the perilipin family. PLIN1 is a hormonally-regulated phosphoprotein that encircles the lipid storage droplet in adipocytes and modulates adipocyte lipid metabolism (PMID: 2040638). PLIN1 is the major cAMP-dependent protein kinase substrate in adipocytes and, when unphosphorylated, may play a role in inhibiting lipolysis. PLIN1 is detected in adipocytes of white adipose tissue, visceral adipose tissue, and mammary gland (PMID: 27832861, 9521880). The deficiency of PLIN1 causes lipodystrophic syndromes, which disables the optimal accumulation of triglycerides in adipocytes that results in abnormal deposition of lipids in tissues such as skeletal muscle and liver. The storage of lipids in the liver leads to insulin resistance and hypertriglyceridemia (PMID: 21345103).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Perilipin 1 antibody 27716-1-AP | Download protocol |
| IHC protocol for Perilipin 1 antibody 27716-1-AP | Download protocol |
| IF protocol for Perilipin 1 antibody 27716-1-AP | Download protocol |
| IP protocol for Perilipin 1 antibody 27716-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Hum Gene Ther Kallistatin Improves High-Fat-Induced Insulin Resistance via Epididymal Adipose Tissue-Derived Exosomes | ||
Metabolism Ubiquitination of cytoplasmic HMGB1 by RNF186 regulates hepatic lipophagy in non-alcoholic fatty liver disease | ||
Cells Development of a Mouse Experimental System for the In Vivo Characterization of Bioengineered Adipose-Derived Stromal Cells |
