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Species Reactivity
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Species Reactivity
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Conjugate
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Conjugate
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Perilipin 1 Recombinant antibody

Perilipin 1 Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

240994H1

Cat no : 83905-4-RR

Synonyms

PLIN, PLIN1, PERI, 240994H1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PLIN1 Recombinant antibody (83905-4-RR)

    WB analysis using 83905-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83905-4-RR (PLIN1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 83905-4-RR

    Affinity and Kinetic Characterization of 83905-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83905-4-RR against Human Perilipin 1 were performed. The affinity constant is 0.617 nM.

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Tested Applications

Positive WB detected inMCF-7 cells, MDA-MB-231 cells, 3T3-L1 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83905-4-RR is Perilipin 1 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Perilipin 1 fusion protein Ag26426 相同性解析による交差性が予測される生物種
Full Name perilipin
Calculated molecular weight 56 kDa
Observed molecular weight67 kDa
GenBank accession numberNM_001145311
Gene symbol Perilipin 1
Gene ID (NCBI) 5346
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PLIN1, also known as Perilipin 1, PLIN, PERI, belongs to the perilipin family. PLIN1 is a hormonally-regulated phosphoprotein that encircles the lipid storage droplet in adipocytes and modulates adipocyte lipid metabolism (PMID: 2040638). PLIN1 is the major cAMP-dependent protein kinase substrate in adipocytes and, when unphosphorylated, may play a role in inhibiting lipolysis. PLIN1 is detected in adipocytes of white adipose tissue, visceral adipose tissue, and mammary gland (PMID: 27832861, 9521880). The deficiency of PLIN1 causes lipodystrophic syndromes, which disables the optimal accumulation of triglycerides in adipocytes that results in abnormal deposition of lipids in tissues such as skeletal muscle and liver. The storage of lipids in the liver leads to insulin resistance and hypertriglyceridemia (PMID: 21345103).

Protocols

Product Specific Protocols
WB protocol for Perilipin 1 antibody 83905-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols