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KD/KO Validated

PLK1 Polyclonal antibody

Name: PLK1 Polyclonal antibody
Brand: Proteintech
SKU: 10305-1-AP

PLK1 Polyclonal Antibody for WB, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, FC (Intra), IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 10305-1-AP

Synonyms

Polo-like kinase 1, Polo like kinase 1, PLK-1, PLK 1, PLK

Validation Data Gallery

Filter:

AllWBIF/ICCFC (Intra)

WB analysis using 10305-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10305-1-AP (PLK1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis using 10305-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10305-1-AP (PLK1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 10305-1-AP
WB result of PLK1 antibody (10305-1-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PLK1 transfected HeLa cells.
WB analysis using 10305-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10305-1-AP (PLK1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis using 10305-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10305-1-AP (PLK1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
IF Staining of U2OS using 10305-1-AP
Immunofluorescent analysis of (4% PFA) fixed U2OS cells using PLK1 antibody (10305-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).
FC experiment of Ramos using 10305-1-AP
1X10^6 Ramos cells were intracellularly stained with 0.4 ug Anti-Human PLK1 (10305-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP, Clone: ) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

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Cat No. 10305-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	HEK-293 cells, A549 cells, HeLa cells, MDA-MB-453s cells, U2OS cells, Jurkat cells, mouse testis tissue, rat testis tissue
Positive IF/ICC detected in	U2OS cells
Positive FC (Intra) detected in	Ramos cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:8000
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 4 publications below
WB	See 31 publications below
IF	See 7 publications below
IP	See 3 publications below
CoIP	See 1 publications below

Product Information

10305-1-AP targets PLK1 in WB, IF/ICC, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	PLK1 fusion protein Ag0284 相同性解析による交差性が予測される生物種
Full Name	polo-like kinase 1 (Drosophila)
Calculated molecular weight	68 kDa
Observed molecular weight	68 kDa
GenBank accession number	BC002369
Gene symbol	PLK1
Gene ID (NCBI)	5347
RRID	AB_2877731
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

PLK1(Polo-like kinase 1), also named as PLK and STPK13, belongs to the protein kinase superfamily, Ser/Thr protein kinase family and CDC5/Polo subfamily. PLK1 is a Serine/threonine-protein kinase that performs several important functions throughout M phase of the cell cycle, including the regulation of centrosome maturation and spindle assembly, the removal of cohesins from chromosome arms, the inactivation of APC/C inhibitors, and the regulation of mitotic exit and cytokinesis.The localization of Polo changes during the cell cycle. In interphase, Polo shows a mostly diffuse cytoplasmic localization. Polo becomes concentrated on centrosomes from early prophase and appears on centromeres or kinetochores from late prophase, before nuclear envelope breakdown (NEB). After anaphase onset, Polo relocalizes to the central spindle and remains enriched at the midbody ring in late stages of cytokinesis (PMID: 29167465).

Protocols

Product Specific Protocols
WB protocol for PLK1 antibody 10305-1-AP	Download protocol
IF protocol for PLK1 antibody 10305-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB,IF	J Clin Invest PLK1 inhibition dampens NLRP3 inflammasome-elicited response in inflammatory disease models Authors - Marta Baldrighi View Article
human	WB	Adv Sci (Weinh) Targeting CBX3 with a Dual BET/PLK1 Inhibitor Enhances the Antitumor Efficacy of CDK4/6 Inhibitors in Prostate Cancer Authors - Huaiyuan Liang KD Validated View Article
human,mouse	WB	Sci Adv Cascade dynamic assembly/disassembly of DNA nanoframework enabling the controlled delivery of CRISPR-Cas9 system Authors - Nachuan Song View Article
human	WB,IF	Nat Commun Hyperactivation of HER2-SHCBP1-PLK1 axis promotes tumor cell mitosis and impairs trastuzumab sensitivity to gastric cancer. Authors - Wengui Shi View Article
human	WB	Biomaterials Biocompatible PEGylated Gold nanorods function As cytokinesis inhibitors to suppress angiogenesis. Authors - Hongyuan Song View Article
rat	WB	Cell Rep Increased degradation of FMRP contributes to neuronal hyperexcitability in tuberous sclerosis complex Authors - Kellen D Winden View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PLK1 Polyclonal antibody

PLK1 Polyclonal Antibody for WB, IF/ICC, FC (Intra), ELISA

Validation Data Gallery

WB analysis using 10305-1-AP

WB analysis using 10305-1-AP

WB analysis of HeLa using 10305-1-AP

WB analysis using 10305-1-AP

WB analysis using 10305-1-AP

IF Staining of U2OS using 10305-1-AP

FC experiment of Ramos using 10305-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

PLK1 inhibition dampens NLRP3 inflammasome-elicited response in inflammatory disease models

Targeting CBX3 with a Dual BET/PLK1 Inhibitor Enhances the Antitumor Efficacy of CDK4/6 Inhibitors in Prostate Cancer

Cascade dynamic assembly/disassembly of DNA nanoframework enabling the controlled delivery of CRISPR-Cas9 system

Hyperactivation of HER2-SHCBP1-PLK1 axis promotes tumor cell mitosis and impairs trastuzumab sensitivity to gastric cancer.

Biocompatible PEGylated Gold nanorods function As cytokinesis inhibitors to suppress angiogenesis.

Increased degradation of FMRP contributes to neuronal hyperexcitability in tuberous sclerosis complex

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