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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PLK1 Recombinant antibody (83260-5-RR)

    WB analysis using 83260-5-RR (same clone as 83260-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83260-5-RR (PLK1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83260-5-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using PLK1 Recombinant antibody (83260-5-RR)

    IF Staining of HeLa using 83260-5-RR (same clone as 83260-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PLK1 antibody (83260-5-RR, Clone: 240092B10 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83260-5-PBS in a different storage buffer formulation.

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PLK1-phospho Recombinant antibody, PBS Only

PLK1-phospho Uni-rAbTM Recombinant Antibody for WB, IF, Indirect ELISA
Cat No. 83260-5-PBS
Clone No.240092B10

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IF, Indirect ELISA

Synonyms

PLK, PLK 1, PLK1, Polo like kinase 1, STPK13

Formulation:  PBS Only
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83260-5-PBS targets PLK1-phospho in WB, IF, Indirect ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name polo-like kinase 1 (Drosophila)
Calculated molecular weight 68 kDa
GenBank accession numberNM_005030
Gene Symbol PLK1
Gene ID (NCBI) 5347
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP53350
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.