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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PLK1 Recombinant antibody

PLK1 Recombinant Antibody for WB, IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IF, ELISA

Conjugate

Unconjugated

CloneNo.

240092B10

Cat no : 83260-5-RR

Synonyms

PLK, PLK 1, PLK1, Polo like kinase 1, STPK13

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PLK1 Recombinant antibody (83260-5-RR)

    WB analysis using 83260-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83260-5-RR (PLK1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using PLK1 Recombinant antibody (83260-5-RR)

    IF Staining of HeLa using 83260-5-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PLK1 antibody (83260-5-RR, Clone: 240092B10 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red).

Tested Applications

Positive WB detected inHEK-293 cells, Jurkat cells
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83260-5-RR targets PLK1 in WB, IF, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name polo-like kinase 1 (Drosophila)
Calculated molecular weight 68 kDa
Observed molecular weight66 kDa
GenBank accession numberNM_005030
Gene symbol PLK1
Gene ID (NCBI) 5347
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PLK1(Polo-like kinase 1), also named as PLK and STPK13, belongs to the protein kinase superfamily, Ser/Thr protein kinase family and CDC5/Polo subfamily. PLK1 is a Serine/threonine-protein kinase that performs several important functions throughout M phase of the cell cycle, including the regulation of centrosome maturation and spindle assembly, the removal of cohesins from chromosome arms, the inactivation of APC/C inhibitors, and the regulation of mitotic exit and cytokinesis.The localization of Polo changes during the cell cycle. In interphase, Polo shows a mostly diffuse cytoplasmic localization. Polo becomes concentrated on centrosomes from early prophase and appears on centromeres or kinetochores from late prophase, before nuclear envelope breakdown (NEB). After anaphase onset, Polo relocalizes to the central spindle and remains enriched at the midbody ring in late stages of cytokinesis (PMID: 29167465).

Protocols

Product Specific Protocols
WB protocol for PLK1 antibody 83260-5-RRDownload protocol
IF protocol for PLK1 antibody 83260-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols