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KD/KO Validated

PLK2 Polyclonal antibody

Name: PLK2 Polyclonal antibody
Brand: Proteintech
SKU: 15956-1-AP

PLK2 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 15956-1-AP

Synonyms

PLK 2, PLK2, Polo like kinase 2, Serum inducible kinase, SNK

Validation Data Gallery

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AllWBIP

WB analysis using 15956-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 15956-1-AP (PLK2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of MCF-7 using 15956-1-AP
MCF-7 cells were subjected to SDS PAGE followed by western blot with 15956-1-AP (PLK2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 15956-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 15956-1-AP (PLK2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
WB analysis of COLO 320 using 15956-1-AP
COLO 320 cells were subjected to SDS PAGE followed by western blot with 15956-1-AP (PLK2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
WB analysis of COLO 320 using 15956-1-AP
COLO 320 cells were subjected to SDS PAGE followed by western blot with 15956-1-AP (PLK2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
IP experiment of HeLa using 15956-1-AP
IP result of anti-PLK2 (IP:15956-1-AP, 4ug; Detection:15956-1-AP 1:300) with HeLa cells lysate 2480ug.

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Cat No. 15956-1-AP

Size/Concentration

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	HeLa cells, MCF-7 cells, COLO 320 cells
Positive IP detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:4000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 2 publications below
IP	See 1 publications below

Product Information

15956-1-AP targets PLK2 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	PLK2 fusion protein Ag8710 相同性解析による交差性が予測される生物種
Full Name	polo-like kinase 2 (Drosophila)
Calculated molecular weight	685 aa, 78 kDa
Observed molecular weight	75-80 kDa
GenBank accession number	BC013879
Gene symbol	PLK2
Gene ID (NCBI)	10769
RRID	AB_2237367
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

PLK2(Polo-like kinase 2) is also named as SNK and belongs to the Ser/Thr protein kinase family. It plays a role in normal cell division. Plk2 expression is detected only in G1, and endogenous Plk2 is rapidly turned over. Therefore, the expression of the three mammalian Plks during the cell cycle resembles the successive transition of the cyclins, prompting speculation that Plks may reg-ulate the cell cycle in a manner analogous to that of Cdks(PMID:12972611).

Protocols

Product Specific Protocols
WB protocol for PLK2 antibody 15956-1-AP	Download protocol
IP protocol for PLK2 antibody 15956-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Oncotarget MicroRNA-27b up-regulated by human papillomavirus 16 E7 promotes proliferation and suppresses apoptosis by targeting polo-like kinase2 in cervical cancer. Authors - Fei Liu View Article
rat	WB	J Biochem Mol Toxicol PLK2 protects retinal ganglion cells from oxidative stress by potentiating Nrf2 signaling via GSK-3β. Authors - Yazhi Fan KD Validated View Article
	IP	Cell Biol Int Tazarotene-induced gene 1 interacts with Polo-like kinase 2 and inhibits cell proliferation in HCT116 colorectal cancer cells. Authors - Chun-Hua Wang View Article

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研究分野

Target

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Species Reactivity

Species Reactivity

Conjugate

Conjugate

PLK2 Polyclonal antibody

PLK2 Polyclonal Antibody for WB, IP, ELISA

Validation Data Gallery

WB analysis using 15956-1-AP

WB analysis of MCF-7 using 15956-1-AP

WB analysis of HeLa using 15956-1-AP

WB analysis of COLO 320 using 15956-1-AP

WB analysis of COLO 320 using 15956-1-AP

IP experiment of HeLa using 15956-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

MicroRNA-27b up-regulated by human papillomavirus 16 E7 promotes proliferation and suppresses apoptosis by targeting polo-like kinase2 in cervical cancer.

PLK2 protects retinal ganglion cells from oxidative stress by potentiating Nrf2 signaling via GSK-3β.

Tazarotene-induced gene 1 interacts with Polo-like kinase 2 and inhibits cell proliferation in HCT116 colorectal cancer cells.