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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PLK3 Polyclonal antibody

PLK3 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Cat no : 30486-1-AP

Synonyms

PLK 3, FNK, FGF-inducible kinase, EC:2.7.11.21, Cytokine-inducible serine/threonine-protein kinase

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PLK3 Polyclonal antibody (30486-1-AP)

    WB analysis using 30486-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30486-1-AP (PLK3 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using PLK3 Polyclonal antibody (30486-1-AP)

    IP experiment of HeLa using 30486-1-AP

    IP result of anti-PLK3 (IP:30486-1-AP, 4ug; Detection:30486-1-AP 1:500) with HeLa cells lysate 1750 ug.

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Tested Applications

Positive WB detected inHEK-293T cells, K-562 cells, NCI-H1299 cells, U2OS cells
Positive IP detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

30486-1-AP targets PLK3 in WB, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PLK3 fusion protein Ag33186 相同性解析による交差性が予測される生物種
Full Name polo-like kinase 3 (Drosophila)
Calculated molecular weight 72 kDa
Observed molecular weight 72 kDa
GenBank accession numberBC013899
Gene symbol PLK3
Gene ID (NCBI) 1263
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PLK3(Polo-like kinase 3) is also named as CNK, FNK, PRK and belongs to the protein kinase superfamily. It is involved in the regulation of DNA damage checkpoint as well as in M-phase function. Plk3 physically interacts with p53 and phosphorylates this tumor suppressor protein on serine-20, suggesting that the role of Plk3 in cell cycle progression is mediated, at least in part, through direct regulation of p53(PMID:12548019).

Protocols

Product Specific Protocols
WB protocol for PLK3 antibody 30486-1-APDownload protocol
IP protocol for PLK3 antibody 30486-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols