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KD/KO Validated

PLK4 Polyclonal antibody

Name: PLK4 Polyclonal antibody
Brand: Proteintech
SKU: 12952-1-AP

PLK4 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 12952-1-AP

Synonyms

PLK 4, PLK4, Polo like kinase 4, SAK, STK18

Validation Data Gallery

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AllWBIHC

WB analysis of A549 cells using 12952-1-AP
WB result of PLK4 antibody (12952-1-AP, 1:2000) with si-control and si-PLK4 transfected A549 cells.
WB analysis using 12952-1-AP
mouse lung tissue were subjected to SDS PAGE followed by western blot with 12952-1-AP (PLK4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of MCF-7 using 12952-1-AP
MCF7 cells were subjected to SDS PAGE followed by western blot with 12952-1-AP (PLK4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of A549 using 12952-1-AP
A549 cells were subjected to SDS PAGE followed by western blot with 12952-1-AP (PLK4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IHC staining of mouse testis using 12952-1-AP
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 12952-1-AP (PLK4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse testis using 12952-1-AP
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 12952-1-AP (PLK4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human cervical cancer using 12952-1-AP
Immunohistochemical analysis of paraffin-embedded human cervical cancer using 12952-1-AP (PLK4 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human cervical cancer using 12952-1-AP
Immunohistochemical analysis of paraffin-embedded human cervical cancer using 12952-1-AP (PLK4 antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human lung cancer using 12952-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer using 12952-1-AP (PLK4 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human lung cancer using 12952-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer using 12952-1-AP (PLK4 antibody) at dilution of 1:50 (under 40x lens).

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Cat No. 12952-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	mouse lung tissue, MCF-7 cells, A549 cells
Positive IHC detected in	mouse testis tissue, human lung cancer tissue, human cervical cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 3 publications below
WB	See 14 publications below
IHC	See 10 publications below
IF	See 6 publications below

Product Information

12952-1-AP targets PLK4 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human, rat, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	PLK4 fusion protein Ag3605 相同性解析による交差性が予測される生物種
Full Name	polo-like kinase 4 (Drosophila)
Calculated molecular weight	970 aa, 109 kDa
Observed molecular weight	105-112 kDa
GenBank accession number	BC036023
Gene symbol	PLK4
Gene ID (NCBI)	10733
RRID	AB_2284150
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

PLK4(Polo-like kinase 4) is also named SAK, STK18 and belongs to the Ser/Thr protein kinase family. It plays a central role in centriole duplication and is a kinase implicated in tumor development, which is an upstream regulator of canonical biogenesis necessary for centriole formation. It has 3 isoforms produced by alternative splicing.

Protocols

Product Specific Protocols
WB protocol for PLK4 antibody 12952-1-AP	Download protocol
IHC protocol for PLK4 antibody 12952-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse, human	WB, IHC	Nat Commun Loss of KLF14 triggers centrosome amplification and tumorigenesis. Authors - Guangjian Fan KO Validated View Article
human	IF	Cancer Res Proteomic mapping and targeting of mitotic pericentriolar material in tumors bearing centrosome amplification. Authors - Bingteng Xie View Article
mouse	IF	EMBO Rep ENKD1 promotes CP110 removal through competing with CEP97 to initiate ciliogenesis. Authors - Ting Song View Article
human	WB,IHC	Cell Death Dis Polo-like kinase 4 mediates epithelial-mesenchymal transition in neuroblastoma via PI3K/Akt signaling pathway. Authors - Xiangdong Tian KD Validated View Article
human	WB	Cell Death Dis NudC-like protein 2 restrains centriole amplification by stabilizing HERC2. Authors - Min Li View Article
human	WB	Mol Cancer Ther Targeting Polo-like Kinase 1 by a Novel Pyrrole-imidazole Polyamide-Hoechst Conjugate Suppresses Tumor Growth in vivo. Authors - Ke Liu View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PLK4 Polyclonal antibody

PLK4 Polyclonal Antibody for WB, IHC, ELISA

Validation Data Gallery

WB analysis of A549 cells using 12952-1-AP

WB analysis using 12952-1-AP

WB analysis of MCF-7 using 12952-1-AP

WB analysis of A549 using 12952-1-AP

IHC staining of mouse testis using 12952-1-AP

IHC staining of mouse testis using 12952-1-AP

IHC staining of human cervical cancer using 12952-1-AP

IHC staining of human cervical cancer using 12952-1-AP

IHC staining of human lung cancer using 12952-1-AP

IHC staining of human lung cancer using 12952-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Loss of KLF14 triggers centrosome amplification and tumorigenesis.

Proteomic mapping and targeting of mitotic pericentriolar material in tumors bearing centrosome amplification.

ENKD1 promotes CP110 removal through competing with CEP97 to initiate ciliogenesis.

Polo-like kinase 4 mediates epithelial-mesenchymal transition in neuroblastoma via PI3K/Akt signaling pathway.

NudC-like protein 2 restrains centriole amplification by stabilizing HERC2.

Targeting Polo-like Kinase 1 by a Novel Pyrrole-imidazole Polyamide-Hoechst Conjugate Suppresses Tumor Growth in vivo.