• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

  • Featured Product
  • KD/KO Validated

PLK4 Polyclonal antibody, PBS Only

PLK4 Polyclonal Antibody for IHC, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 28750-1-PBS

Synonyms

Validation Data Gallery

Filter:
  • Immunohistochemistry (IHC) staining of human colon cancer tissue using PLK4 Polyclonal antibody (28750-1-AP)

    IHC staining of human colon cancer using 28750-1-AP (same clone as 28750-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 28750-1-AP (PLK4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28750-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using PLK4 Polyclonal antibody (28750-1-AP)

    IHC staining of human colon cancer using 28750-1-AP (same clone as 28750-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 28750-1-AP (PLK4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28750-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using PLK4 Polyclonal antibody (28750-1-AP)

    IHC staining of mouse testis using 28750-1-AP (same clone as 28750-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 28750-1-AP (PLK4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28750-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using PLK4 Polyclonal antibody (28750-1-AP)

    IHC staining of mouse testis using 28750-1-AP (same clone as 28750-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 28750-1-AP (PLK4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28750-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of hTERT-RPE1 cells using PLK4 Polyclonal antibody (28750-1-AP)

    IF Staining of hTERT-RPE1 using 28750-1-AP (same clone as 28750-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed hTERT-RPE1 cells using PLK4 antibody (28750-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CoraLite®594 ARL13B antibody (CL594-17711, red). This data was developed using the same antibody clone with 28750-1-PBS in a different storage buffer formulation.

☆対象製品が30%オフ！組換えタンパク質&免染用試薬 >>> キャンペーン実施中！

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

28750-1-PBS targets PLK4 in IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PLK4 fusion protein Ag30482 相同性解析による交差性が予測される生物種
Full Name polo-like kinase 4 (Drosophila)
Calculated molecular weight 970 aa, 109 kDa
GenBank accession numberBC036023
Gene symbol PLK4
Gene ID (NCBI) 10733
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

PLK4 (Polo-like kinase 4) is also named SAK, STK18 and belongs to the Ser/Thr protein kinase family. It plays a central role in centriole duplication and is a kinase implicated in tumor development, which is an upstream regulator of canonical biogenesis necessary for centriole formation. It has 3 isoforms produced by alternative splicing.