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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PLK5 Polyclonal antibody

PLK5 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 21299-1-AP

Synonyms

PLK5P, polo like kinase 5 pseudogene

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using PLK5 Polyclonal antibody (21299-1-AP)

    WB analysis using 21299-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21299-1-AP (PLK5 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse testis tissue using PLK5 Polyclonal antibody (21299-1-AP)

    WB analysis of mouse testis using 21299-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 21299-1-AP (PLK5 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human brain tissue using PLK5 Polyclonal antibody (21299-1-AP)

    IHC staining of human brain using 21299-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 21299-1-AP (PLK5 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human brain tissue using PLK5 Polyclonal antibody (21299-1-AP)

    IHC staining of human brain using 21299-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 21299-1-AP (PLK5 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using PLK5 Polyclonal antibody (21299-1-AP)

    IHC staining of human testis using 21299-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 21299-1-AP (PLK5 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using PLK5 Polyclonal antibody (21299-1-AP)

    IHC staining of human testis using 21299-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 21299-1-AP (PLK5 antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using PLK5 Polyclonal antibody (21299-1-AP)

    IF Staining of HeLa using 21299-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PLK5 antibody (21299-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inK-562 cells, mouse testis tissue, mouse kidney tissue
Positive IHC detected inhuman brain tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

21299-1-AP targets PLK5 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PLK5 fusion protein Ag15881 相同性解析による交差性が予測される生物種
Full Name polo-like kinase 5 pseudogene
Calculated molecular weight 336 aa, 36 kDa
Observed molecular weight 55-60 kDa
GenBank accession numberBC100792
Gene symbol PLK5
Gene ID (NCBI) 126520
RRIDAB_10732958
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PLK5, also named as PLK5P, is an inactive serine/threonine-protein kinase that plays a role in cell cycle progression and neuronal differentiation. Compared with mouse Plk5, the protein kinase domain of human PLK5 is truncated. This is the reason that the MW of mouse Plk5 is about 50-70 kDa, the MW of human PLK5 is about 32-40 kDa. PLK5 is located in the cytoplasm and nucleus(Uniprot). This antibody detects both PLK5 gene of human and mouse.

Protocols

Product Specific Protocols
WB protocol for PLK5 antibody 21299-1-APDownload protocol
IHC protocol for PLK5 antibody 21299-1-APDownload protocol
IF protocol for PLK5 antibody 21299-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC,IF

Eur J Cell Biol

TWEAK/Fn14 signaling may function as a reactive compensatory mechanism against extracellular matrix accumulation in keloid fibroblasts

Authors - Yitian Zhang
View Article