Validation Data Gallery
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Tested Applications
| Positive WB detected in | K-562 cells, mouse testis tissue, mouse kidney tissue |
| Positive IHC detected in | human brain tissue, human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
| IHC | See 1 publications below |
| IF | See 2 publications below |
Product Information
21299-1-AP targets PLK5 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | PLK5 fusion protein Ag15881 相同性解析による交差性が予測される生物種 |
| Full Name | polo-like kinase 5 pseudogene |
| Calculated molecular weight | 336 aa, 36 kDa |
| Observed molecular weight | 55-60 kDa |
| GenBank accession number | BC100792 |
| Gene Symbol | PLK5 |
| Gene ID (NCBI) | 126520 |
| RRID | AB_10732958 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q496M5 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
PLK5, also named as PLK5P, is an inactive serine/threonine-protein kinase that plays a role in cell cycle progression and neuronal differentiation. Compared with mouse Plk5, the protein kinase domain of human PLK5 is truncated. This is the reason that the MW of mouse Plk5 is about 50-70 kDa, the MW of human PLK5 is about 32-40 kDa. PLK5 is located in the cytoplasm and nucleus(Uniprot). This antibody detects both PLK5 gene of human and mouse.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for PLK5 antibody 21299-1-AP | Download protocol |
| IHC protocol for PLK5 antibody 21299-1-AP | Download protocol |
| IF protocol for PLK5 antibody 21299-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Eur J Cell Biol TWEAK/Fn14 signaling may function as a reactive compensatory mechanism against extracellular matrix accumulation in keloid fibroblasts | ||
Hum Mol Genet Rare and common genetic variants underlying the risk of Hirschsprung's disease |