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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PLOD1 Polyclonal antibody (29480-1-AP)

    WB analysis using 29480-1-AP

    A375 cells were subjected to SDS PAGE followed by western blot with 29480-1-AP (PLOD1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using PLOD1 Polyclonal antibody (29480-1-AP)

    WB analysis using 29480-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29480-1-AP (PLOD1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using PLOD1 Polyclonal antibody (29480-1-AP)

    WB analysis using 29480-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 29480-1-AP (PLOD1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using PLOD1 Polyclonal antibody (29480-1-AP)

    IHC staining of human skin cancer using 29480-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 29480-1-AP (PLOD1 antibody) at dilution of 1:200 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using PLOD1 Polyclonal antibody (29480-1-AP)

    IHC staining of human skin cancer using 29480-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 29480-1-AP (PLOD1 antibody) at dilution of 1:200 (under 10x lens).

  • Immunofluorescence (IF) / fluorescent staining of K-562 cells using PLOD1 Polyclonal antibody (29480-1-AP)

    IF Staining of K-562 using 29480-1-AP

    Immunofluorescent analysis of (4% PFA) fixed K-562 cells using PLOD1 antibody (29480-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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PLOD1 Polyclonal antibody

PLOD1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 29480-1-AP
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

Human and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

FLJ42041, LH, LH1, LLH, Lysyl hydroxylase 1, PLOD, PLOD1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inA375 cells, K-562 cells, HeLa cells, U-251 cells, U2OS cells
Positive IHC detected inhuman skin cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inK-562 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IHCSee 1 publications below

Product Information

29480-1-AP targets PLOD1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PLOD1 fusion protein Ag31626 相同性解析による交差性が予測される生物種
Full Name procollagen-lysine 1, 2-oxoglutarate 5-dioxygenase 1
Calculated molecular weight 727 aa, 84 kDa
Observed molecular weight84-88 kDa
GenBank accession numberBC016657
Gene Symbol PLOD1
Gene ID (NCBI) 5351
RRIDAB_2918315
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ02809
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PLOD1, as known as procollagen‐lysine, 2‐oxoglutarate 5‐dioxygenase 1, is a member of the PLOD family(Uniprot, GeneID:5351). PLOD1 participates in catalyzing hydroxylysine residue, which is critical for the formation of covalent cross-link (PMID:30003082). PLOD1 plays an important role in the development and progression of tumors. Aberrantly expressed PLOD1 promotes cancer aggressiveness in glioma and bladder cancer, providing a potential prognostic biomarker and therapeutic target (PMID:31199049, PMID:34040895). PLOD1 has 2 isoforms produced by alternative splicing.

Protocols

Product Specific Protocols
WB protocol for PLOD1 antibody 29480-1-APDownload protocol
IHC protocol for PLOD1 antibody 29480-1-APDownload protocol
IF protocol for PLOD1 antibody 29480-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Aging (Albany NY)

Systematic characterization of the expression, prognosis and immune characteristics of PLOD family genes in breast cancer

Authors - Dan-Dan Wang
View Article