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Validation Data Gallery

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  • Western Blot (WB) analysis of THLE-2 cells using PLOD2 Monoclonal antibody (66342-1-Ig)

    WB analysis of THLE-2 using 66342-1-Ig (same clone as 66342-1-PBS)

    WB result of PLOD2 antibody (66342-1-Ig; 1:15000; room temperature for 1.5 hours) with wild-type and PLOD2 knockout THLE-2 cells. This data was developed using the same antibody clone with 66342-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using PLOD2 Monoclonal antibody (66342-1-Ig)

    WB analysis using 66342-1-Ig (same clone as 66342-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66342-1-Ig (PLOD2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66342-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using PLOD2 Monoclonal antibody (66342-1-Ig)

    WB analysis of HEK-293 using 66342-1-Ig (same clone as 66342-1-PBS)

    WB result of PLOD2 antibody (66342-1-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PLOD2 transfected HEK-293 cells. This data was developed using the same antibody clone with 66342-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of DU 145 cells using PLOD2 Monoclonal antibody (66342-1-Ig)

    WB analysis of DU 145 using 66342-1-Ig (same clone as 66342-1-PBS)

    DU 145 cells were subjected to SDS PAGE followed by western blot with 66342-1-Ig (PLOD2 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66342-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A431 cells using PLOD2 Monoclonal antibody (66342-1-Ig)

    WB analysis of A431 using 66342-1-Ig (same clone as 66342-1-PBS)

    A431 cells were subjected to SDS PAGE followed by western blot with 66342-1-Ig (PLOD2 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66342-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using PLOD2 Monoclonal antibody (66342-1-Ig)

    IHC staining of human liver cancer using 66342-1-Ig (same clone as 66342-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66342-1-Ig (PLOD2 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66342-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using PLOD2 Monoclonal antibody (66342-1-Ig)

    IHC staining of human liver cancer using 66342-1-Ig (same clone as 66342-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66342-1-Ig (PLOD2 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66342-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using PLOD2 Monoclonal antibody (66342-1-Ig)

    IHC staining of human liver cancer using 66342-1-Ig (same clone as 66342-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66342-1-Ig (PLOD2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66342-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using PLOD2 Monoclonal antibody (66342-1-Ig)

    IHC staining of human liver cancer using 66342-1-Ig (same clone as 66342-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66342-1-Ig (PLOD2 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66342-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

PLOD2 Monoclonal antibody, PBS Only

PLOD2 Monoclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 66342-1-PBS
Clone No.1H9E1

Host / Isotype

Mouse / IgA

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Synonyms

Procollagen-lysine,2-oxoglutarate 5-dioxygenase 2, PLOD2/LH2, Lysyl hydroxylase 2, EC:1.14.11.4, 1H9E1

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Mouse monoclonal antibodies of IgA isotype can be detected with "anti-mouse IgG (H+L)" secondary antibodies.

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66342-1-PBS targets PLOD2 in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgA
Class Monoclonal
Type Antibody
Immunogen PLOD2 fusion protein Ag5779 相同性解析による交差性が予測される生物種
Full Name procollagen-lysine, 2-oxoglutarate 5-dioxygenase 2
Calculated molecular weight 758 aa, 85 kDa
Observed molecular weight 87 kDa
GenBank accession numberBC037169
Gene Symbol PLOD2
Gene ID (NCBI) 5352
RRIDAB_2881722
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO00469
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

PLOD2,also named as LH2, forms hydroxylysine residues in -Xaa-Lys-Gly- sequences in collagens. It is a potential novel prognostic factor for HCC patients following surgery. Among the PLOD genes, PLOD2 contributes to cancer prognosis and angiogenesis. Several authors have reported that PLOD2 expression might provide prognostic information about malignant tumours such as glioblastoma. PLOD2 expression is a useful biomarker for the effects of antiangiogenic treatment for malignancy.(PMID:22098155). It has 2 isoforms produced by alternative splicing and seven glycosylation sites.