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  • KD/KO Validated

CoraLite®594-conjugated PLOD3 Monoclonal antibody

PLOD3 Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

IF/ICC

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

2G12B8

Cat no : CL594-60058

Synonyms

Procollagen galactosyltransferase, Multifunctional procollagen lysine hydroxylase and glycosyltransferase LH3, Lysyl hydroxylase 3, LH3, Galactosylhydroxylysine-glucosyltransferase

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite®594-conjugated PLOD3 Monoclonal antibody (CL594-60058)

    IF Staining of HeLa using CL594-60058

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CL594-60058 (PLOD3 antibody) at dilution of 1:50.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite®594-conjugated PLOD3 Monoclonal antibody (CL594-60058)

    IF Staining of HeLa using CL594-60058

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CoraLite®594 PLOD3 antibody (CL594-60058, Clone: 2G12B8 ) at dilution of 1:200.

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Tested Applications

Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-60058 targets PLOD3 in IF/ICC applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen PLOD3 fusion protein Ag1480 相同性解析による交差性が予測される生物種
Full Name procollagen-lysine, 2-oxoglutarate 5-dioxygenase 3
Calculated molecular weight 738 aa, 85 kDa
Observed molecular weight 80-85 kDa
GenBank accession numberBC011674
Gene symbol PLOD3
Gene ID (NCBI) 8985
RRIDAB_2883412
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PLOD3, also named as LH3, forms hydroxylysine residues in -Xaa-Lys-Gly- sequences in collagens. These hydroxylysines serve as sites of attachment for carbohydrate units and are essential for the stability of the intermolecular collagen cross-links. The major function of PLOD3 in osteoblasts is to glucosylate galactosylhydroxylysine residues in type I collagen.

Protocols

Product Specific Protocols
IF protocol for CL594 PLOD3 antibody CL594-60058Download protocol
Standard Protocols
Click here to view our Standard Protocols