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KD/KO Validated

PLS1 Polyclonal antibody

Name: PLS1 Polyclonal antibody
Brand: Proteintech
SKU: 55212-1-AP

PLS1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 55212-1-AP

Synonyms

Plastin-1, Plastin 1, I-plastin, Intestine-specific plastin, I plastin

Validation Data Gallery

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AllWBIHC

WB analysis of mouse small intestine using 55212-1-AP
mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 55212-1-AP (PLS1 antibody) at dilution of 1:1200 incubated at room temperature for 1.5 hours.
WB analysis of COLO 320 using 55212-1-AP
COLO 320 cells were subjected to SDS PAGE followed by western blot with 55212-1-AP (PLS1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of COLO 320 using 55212-1-AP
COLO 320 cells were subjected to SDS PAGE followed by western blot with 55212-1-AP (PLS1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse small intestine using 55212-1-AP
mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 55212-1-AP (PLS1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse small intestine using 55212-1-AP
mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 55212-1-AP (PLS1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of COLO 320 using 55212-1-AP
COLO 320 cells were subjected to SDS PAGE followed by western blot with 55212-1-AP (PLS1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of COLO 320 using 55212-1-AP
COLO 320 cells were subjected to SDS PAGE followed by western blot with 55212-1-AP (PLS1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IHC staining of human colon cancer using 55212-1-AP
Immunohistochemical analysis of paraffin-embedded colon cancer slide using 55212-1-AP (PLS1 antibody) at dilution of 1:300 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human kidney using 55212-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney using 55212-1-AP (PLS1 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human kidney using 55212-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney using 55212-1-AP (PLS1 antibody) at dilution of 1:50 (under 40x lens).

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Cat No. 55212-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	mouse small intestine tissue, COLO 320 cells
Positive IHC detected in	human colon cancer tissue, human kidney tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2400
Immunohistochemistry (IHC)	IHC : 1:150-1:600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 1 publications below
IHC	See 1 publications below

Product Information

55212-1-AP targets PLS1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Peptide 相同性解析による交差性が予測される生物種
Full Name	plastin 1 (I isoform)
Calculated molecular weight	70 kDa
Observed molecular weight	70 kDa
GenBank accession number	NM_002670
Gene symbol	PLS1
Gene ID (NCBI)	5357
RRID	AB_10859080
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

PLS1, also named as I-plastin and Plastin-1, is an actin-bundling protein in the absence of calcium. It is intestine-specific plastin. This antibody is specific to PLS1. This antibody is specific to PLS1. It has no cross reaction to PLS3.

Protocols

Product Specific Protocols
WB protocol for PLS1 antibody 55212-1-AP	Download protocol
IHC protocol for PLS1 antibody 55212-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB,IHC	Cancer Sci Plastin 1 drives metastasis of colorectal cancer through the IQGAP1/Rac1/ERK pathway. Authors - Tongtong Zhang KD Validated View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PLS1 Polyclonal antibody

PLS1 Polyclonal Antibody for WB, IHC, ELISA

Validation Data Gallery

WB analysis of mouse small intestine using 55212-1-AP

WB analysis of COLO 320 using 55212-1-AP

WB analysis of COLO 320 using 55212-1-AP

WB analysis of mouse small intestine using 55212-1-AP

WB analysis of mouse small intestine using 55212-1-AP

WB analysis of COLO 320 using 55212-1-AP

WB analysis of COLO 320 using 55212-1-AP

IHC staining of human colon cancer using 55212-1-AP

IHC staining of human kidney using 55212-1-AP

IHC staining of human kidney using 55212-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Plastin 1 drives metastasis of colorectal cancer through the IQGAP1/Rac1/ERK pathway.