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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse small intestine tissue using PLS1 Polyclonal antibody (55212-1-AP)

    WB analysis of mouse small intestine using 55212-1-AP

    mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 55212-1-AP (PLS1 antibody) at dilution of 1:1200 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of COLO 320 cells using PLS1 Polyclonal antibody (55212-1-AP)

    WB analysis of COLO 320 using 55212-1-AP

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 55212-1-AP (PLS1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of COLO 320 cells using PLS1 Polyclonal antibody (55212-1-AP)

    WB analysis of COLO 320 using 55212-1-AP

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 55212-1-AP (PLS1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of COLO 320 cells using PLS1 Polyclonal antibody (55212-1-AP)

    WB analysis of COLO 320 using 55212-1-AP

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 55212-1-AP (PLS1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of COLO 320 cells using PLS1 Polyclonal antibody (55212-1-AP)

    WB analysis of COLO 320 using 55212-1-AP

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 55212-1-AP (PLS1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse small intestine tissue using PLS1 Polyclonal antibody (55212-1-AP)

    WB analysis of mouse small intestine using 55212-1-AP

    mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 55212-1-AP (PLS1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse small intestine tissue using PLS1 Polyclonal antibody (55212-1-AP)

    WB analysis of mouse small intestine using 55212-1-AP

    mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 55212-1-AP (PLS1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using PLS1 Polyclonal antibody (55212-1-AP)

    IHC staining of human colon cancer using 55212-1-AP

    Immunohistochemical analysis of paraffin-embedded colon cancer slide using 55212-1-AP (PLS1 antibody) at dilution of 1:300 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human kidney tissue using PLS1 Polyclonal antibody (55212-1-AP)

    IHC staining of human kidney using 55212-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 55212-1-AP (PLS1 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using PLS1 Polyclonal antibody (55212-1-AP)

    IHC staining of human kidney using 55212-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 55212-1-AP (PLS1 antibody) at dilution of 1:50 (under 40x lens).

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  • Featured Product
  • KD/KO Validated

PLS1 Polyclonal antibody

PLS1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 55212-1-AP
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

Plastin-1, Plastin 1, I-plastin, Intestine-specific plastin, I plastin

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse small intestine tissue, COLO 320 cells
Positive IHC detected inhuman colon cancer tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2400
Immunohistochemistry (IHC)IHC : 1:150-1:600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

55212-1-AP targets PLS1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name plastin 1 (I isoform)
Calculated molecular weight 70 kDa
Observed molecular weight 70 kDa
GenBank accession numberNM_002670
Gene Symbol PLS1
Gene ID (NCBI) 5357
RRIDAB_10859080
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ14651
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PLS1, also named as I-plastin and Plastin-1, is an actin-bundling protein in the absence of calcium. It is intestine-specific plastin. This antibody is specific to PLS1. This antibody is specific to PLS1. It has no cross reaction to PLS3.

Protocols

Product Specific Protocols
WB protocol for PLS1 antibody 55212-1-APDownload protocol
IHC protocol for PLS1 antibody 55212-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC

Cancer Sci

Plastin 1 drives metastasis of colorectal cancer through the IQGAP1/Rac1/ERK pathway.

Authors - Tongtong Zhang
  • KD Validated
View Article