• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PLS3 Polyclonal antibody

PLS3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 12917-1-AP

Synonyms

T-plastin, T-fimbrin, T plastin, Plastin-3, Plastin 3

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using PLS3 Polyclonal antibody (12917-1-AP)

    WB analysis using 12917-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12917-1-AP (PLS3 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A431 cells using PLS3 Polyclonal antibody (12917-1-AP)

    WB analysis of A431 using 12917-1-AP

    A431 cells were subjected to SDS PAGE followed by western blot with 12917-1-AP (PLS3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of A431 cells using PLS3 Polyclonal antibody (12917-1-AP)

    IP experiment of A431 using 12917-1-AP

    IP result of anti-PLS3 (IP:12917-1-AP, 4ug; Detection:12917-1-AP 1:500) with A431 cells lysate 2640 ug.

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using PLS3 Polyclonal antibody (12917-1-AP)

    IHC staining of human skin cancer using 12917-1-AP

    Immunohistochemical analysis of paraffin-embedded skin cancer slide using 12917-1-AP (PLS3 antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using PLS3 Polyclonal antibody (12917-1-AP)

    IHC staining of human skin cancer using 12917-1-AP

    Immunohistochemical analysis of paraffin-embedded skin cancer slide using 12917-1-AP (PLS3 antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using PLS3 Polyclonal antibody (12917-1-AP)

    IF Staining of A431 using 12917-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed A431 cells using PLS3 antibody (12917-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using PLS3 Polyclonal antibody (12917-1-AP)

    IF Staining of A431 using 12917-1-AP

    Immunofluorescent analysis of A431 cells using 12917-1-AP (PLS3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

☆大好評トライアルサイズ ¥20,000～！ >>> 一次抗体特別価格キャンペーン開催中！

Tested Applications

Positive WB detected inA431 cells, HEK-293 cells, HeLa cells, HepG2 cells, NIH/3T3 cells
Positive IP detected inA431 cells
Positive IHC detected inhuman skin cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:400-1:1600
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

12917-1-AP targets PLS3 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PLS3 fusion protein Ag3574 相同性解析による交差性が予測される生物種
Full Name plastin 3 (T isoform)
Calculated molecular weight 630 aa, 71 kDa
Observed molecular weight 68 kDa
GenBank accession numberBC039049
Gene symbol PLS3
Gene ID (NCBI) 5358
RRIDAB_2877892
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PLS3, also named T-plastin and Plastin-3, is an actin-bundling protein found in intestinal microvilli, hair cell stereocilia, and fibroblast filopodia. It plays an important role in leukocyte function. L-plastin plays an important role in leukocyte function, and T-plastin is possibly involved in DNA repair. Both T- and L-plastin are implicated in several diseases, and L-plastin is considered to be a valuable marker for cancer (PMID: 15960882).

Protocols

Product Specific Protocols
WB protocol for PLS3 antibody 12917-1-APDownload protocol
IHC protocol for PLS3 antibody 12917-1-APDownload protocol
IF protocol for PLS3 antibody 12917-1-APDownload protocol
IP protocol for PLS3 antibody 12917-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Nat Commun

Cul3 regulates cytoskeleton protein homeostasis and cell migration during a critical window of brain development.

Authors - Jasmin Morandell
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Gaurav (Verified Customer) (02-06-2024)

Excellent antibody with expected molecular weight at right position. Works very well in knock out samples of Smooth Muscle Cells. Works even better at room temperature also. Very stable antibody.

  • Applications: Western Blot, Immunoprecipitation, Cell culture
  • Primary Antibody Dilution: 1:5000
  • Cell Tissue Type: Aorta, Placenta
PLS3 Antibody Western Blot,Immunoprecipitation,Cell culture validation (1:5000 dilution) in Aorta, Placenta (Cat no:12917-1-AP)