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- KD/KO Validated
PLS3 Polyclonal antibody
PLS3 Polyclonal Antibody for WB, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, ELISA
Conjugate
Unconjugated
Cat no : 55216-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HepG2 cells, COLO 320 cells, HeLa cells |
Positive IHC detected in | human oesophagus tissue, human skeletal muscle tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:1000 |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
KD/KO | See 3 publications below |
WB | See 4 publications below |
IHC | See 2 publications below |
Product Information
55216-1-AP targets PLS3 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | Peptide 相同性解析による交差性が予測される生物種 |
Full Name | plastin 3 (T isoform) |
Calculated molecular weight | 71 kDa |
Observed molecular weight | 63 kDa, 38 kDa |
GenBank accession number | NM_005032 |
Gene symbol | PLS3 |
Gene ID (NCBI) | 5358 |
RRID | AB_10858397 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
PLS3, also named as T-plastin and Plastin-3, is an actin-bundling protein found in intestinal microvilli, hair cell stereocilia, and fibroblast filopodia. It plays an important role in leukocyte function. Both T- and L-plastin are implicated in several diseases, L-plastin is considered to be a valuable marker for cancer. (PMID: 15960882) This antibody is specific to PLS3
Protocols
Product Specific Protocols | |
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WB protocol for PLS3 antibody 55216-1-AP | Download protocol |
IHC protocol for PLS3 antibody 55216-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
J Cell Physiol PLS3 predicts poor prognosis in pancreatic cancer and promotes cancer cell proliferation via PI3K/AKT signaling.
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Environ Toxicol PLS3 promotes papillary thyroid carcinoma progression by activating the Notch signaling pathway
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J Cell Biochem Phosphorylation of mitochondrial phospholipid scramblase 3 by protein kinase C-delta induces its activation and facilitates mitochondrial targeting of tBid.
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Biochim Biophys Acta Comparative proteomic analysis of mouse models of pathological and physiological cardiac hypertrophy, with selection of biomarkers of pathological hypertrophy by integrative Proteogenomics. |