Validation Data Gallery

Filter: AllWBIHC

WB analysis of HepG2 using 55216-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 55216-1-AP (PLS3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of COLO 320 using 55216-1-AP
COLO 320 cells were subjected to SDS PAGE followed by western blot with 55216-1-AP (PLS3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis of COLO 320 using 55216-1-AP
COLO 320 cells were subjected to SDS PAGE followed by western blot with 55216-1-AP (PLS3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 55216-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 55216-1-AP (PLS3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 55216-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 55216-1-AP (PLS3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 55216-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 55216-1-AP (PLS3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IHC staining of human oesophagus using 55216-1-AP
Immunohistochemical analysis of paraffin-embedded human oesophagus using 55216-1-AP (PLS3 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human oesophagus using 55216-1-AP
Immunohistochemical analysis of paraffin-embedded human oesophagus using 55216-1-AP (PLS3 antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human skeletal muscle using 55216-1-AP
Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 55216-1-AP (PLS3 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human skeletal muscle using 55216-1-AP
Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 55216-1-AP (PLS3 antibody) at dilution of 1:50 (under 40x lens).

Featured Product
KD/KO Validated

PLS3 Polyclonal antibody

PLS3 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 55216-1-AP

Publications(4)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

T-plastin, T-fimbrin, T plastin, Plastin-3, Plastin 3

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (4)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HepG2 cells, COLO 320 cells, HeLa cells
Positive IHC detected in	human oesophagus tissue, human skeletal muscle tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunohistochemistry (IHC)	IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 3 publications below
WB	See 4 publications below
IHC	See 2 publications below

Product Information

55216-1-AP targets PLS3 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Peptide 相同性解析による交差性が予測される生物種
Full Name	plastin 3 (T isoform)
Calculated molecular weight	71 kDa
Observed molecular weight	63 kDa, 38 kDa
GenBank accession number	NM_005032
Gene Symbol	PLS3
Gene ID (NCBI)	5358
RRID	AB_10858397
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P13797
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

PLS3, also named as T-plastin and Plastin-3, is an actin-bundling protein found in intestinal microvilli, hair cell stereocilia, and fibroblast filopodia. It plays an important role in leukocyte function. Both T- and L-plastin are implicated in several diseases, L-plastin is considered to be a valuable marker for cancer. (PMID: 15960882) This antibody is specific to PLS3

Protocols

Product Specific Protocols
WB protocol for PLS3 antibody 55216-1-AP	Download protocol
IHC protocol for PLS3 antibody 55216-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB,IHC	J Cell Physiol PLS3 predicts poor prognosis in pancreatic cancer and promotes cancer cell proliferation via PI3K/AKT signaling. Authors - Zechang Xin KD Validated View Article
human	WB,IHC	Environ Toxicol PLS3 promotes papillary thyroid carcinoma progression by activating the Notch signaling pathway Authors - Dongtao Wang KD Validated View Article
human	WB	J Cell Biochem Phosphorylation of mitochondrial phospholipid scramblase 3 by protein kinase C-delta induces its activation and facilitates mitochondrial targeting of tBid. Authors - Yongwen He KD Validated View Article
mouse	WB	Biochim Biophys Acta Comparative proteomic analysis of mouse models of pathological and physiological cardiac hypertrophy, with selection of biomarkers of pathological hypertrophy by integrative Proteogenomics. Authors - Hye Kyeong Kwon View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of HepG2 using 55216-1-AP

WB analysis of COLO 320 using 55216-1-AP

WB analysis of COLO 320 using 55216-1-AP

WB analysis of HeLa using 55216-1-AP