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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PLSCR1 Recombinant antibody (84888-4-RR)

    WB analysis using 84888-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84888-4-RR (PLSCR1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 84888-4-RR

    Affinity and Kinetic Characterization of 84888-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84888-4-RR against Human PLSCR1 were performed. The affinity constant is 0.377 nM.

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PLSCR1 Recombinant antibody

PLSCR1 Recombinant Antibody for WB, ELISA
Cat No. 84888-4-RR
Clone No.242372B2

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

phospholipid scramblase 1, MMTRA1B, Mg(2+)-dependent nuclease, EC:3.1.-.-, Ca(2+)-dependent phospholipid scramblase 1

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHepG2 cells, HEK-293 cells, A549 cells, MCF-7 cells, Jurkat cells, human placenta tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84888-4-RR targets PLSCR1 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen PLSCR1 fusion protein Ag2172 相同性解析による交差性が予測される生物種
Full Name phospholipid scramblase 1
Calculated molecular weight 318 aa, 35 kDa
Observed molecular weight34 kDa
GenBank accession numberBC021100
Gene Symbol PLSCR1
Gene ID (NCBI) 5359
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDO15162
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PLSCR1 (Phospholipid scramblase 1) is a member of the family of membrane proteins that mediate the transbilayer movement of phospholipids ("scrambling") in a Ca2+ dependent manner, which was identified as an a type II transmembrane protein involved in the calcium-dependent, nonspecific, rapid redistribution of phospholipids. A cysteine-rich palmitoylation motif [184CCCPCC189] of PLSCR1, a thioester linkage to the sulfhydryl groups of cysteines, controls the transportation of PLSCR1 to the cell membrane or nucleus (PMID: 32292520). PLSCR1 is strongly expressed in response to interferon (IFN) treatment and viral infection (PMID: 35138119, 23487022).

Protocols

Product Specific Protocols
WB protocol for PLSCR1 antibody 84888-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols