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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Anti-Mouse PLVAP (MECA-32)

PLVAP Monoclonal Antibody for IF-P, FC

Host / Isotype

Rat / IgG2a, kappa

Reactivity

Mouse

Applications

IF-P, FC

Conjugate

Unconjugated

CloneNo.

MECA-32

Cat no : 65214-1-Ig

Synonyms

MECA-32, Panendothelial cell antigen, Plasmalemma vesicle protein 1, Plvap, PV 1

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of mouse small intestine tissue using Anti-Mouse PLVAP (MECA-32) (65214-1-Ig)

    IF Staining of mouse small intestine using 65214-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse small intestine tissue using PLVAP antibody (65214-1-Ig, Clone: MECA-32 ) at dilution of 1:250 and Rhodamine (TRITC)-conjugated Goat Anti-Rat IgG(H+L). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Flow cytometry (FC) experiment of bEnd.3 cells using Anti-Mouse PLVAP (MECA-32) (65214-1-Ig)

    FC experiment of bEnd.3 using 65214-1-Ig

    1X10^6 bEnd.3 cells were surface stained with 0.5 ug Anti-Mouse Plvap (65214-1-Ig, Clone:MECA-32) (red), or 0.5 ug Isotype Control. Cells were not fixed.

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Tested Applications

Positive IF-P detected inmouse small intestine tissue
Positive FC detected inbEnd.3 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:125-1:500
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

65214-1-Ig targets PLVAP in IF-P, FC applications and shows reactivity with Mouse samples.

Tested Reactivity Mouse
Host / Isotype Rat / IgG2a, kappa
Class Monoclonal
Type Antibody
Immunogen Mouse lymph node stromal cells 相同性解析による交差性が予測される生物種
Full Name plasmalemma vesicle associated protein
GenBank accession numberBC013517
Gene symbol Plvap
Gene ID (NCBI) 84094
RRIDAB_3084905
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.09% sodium azide.
Storage ConditionsStore at 2-8°C. Stable for one year after shipment.

Background Information

Plasmalemma vesicle-associated protein (PLVAP), also known as PV-1, PAL-E and MECA-32 antigen, is an endothelial cell-specific type II membrane N-glycosylated glycoprotein with a molecular weight of approximately 50-65 kDa, that forms homodimers in situ (PMID: 7626790; 30231925). The MECA-32 antibody, derived from rats immunized with murine lymph node stroma, shows high specificity for mouse endothelium in both embryonic and mature tissues (PMID: 2683264; 7626790). It recognizes most endothelial cells in the embryonic and in the adult mouse, with the exception of the brain, skeletal, and cardiac muscle, where the MECA-32 antigen has a more restricted distribution. In skeletal and cardiac muscle only small arterioles and venules express the MECA-32 antigen, while in the brain its expression is negatively correlated with the differentiation of the vasculature to form the blood brain barrier (PMID: 7626790).

Protocols

Product Specific Protocols
IF protocol for PLVAP antibody 65214-1-IgDownload protocol
FC protocol for PLVAP antibody 65214-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols