Validation Data Gallery

Filter: AllWBIHC

WB analysis of A431 using 12285-1-AP
A431 cells were subjected to SDS PAGE followed by western blot with 12285-1-AP (PLXDC2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
WB analysis using 12285-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 12285-1-AP (PLXDC2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 12285-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 12285-1-AP (PLXDC2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IHC staining of human lung cancer using 12285-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12285-1-AP (PLXDC2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung cancer using 12285-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12285-1-AP (PLXDC2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 12285-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12285-1-AP (PLXDC2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 12285-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12285-1-AP (PLXDC2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

PLXDC2 Polyclonal antibody

PLXDC2 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 12285-1-AP

Publications(3)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Synonyms

UNQ2514/PRO6003, Tumor endothelial marker 7-related protein, TEM7R, Plexin domain-containing protein 2, plexin domain containing 2

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (3)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	A431 cells, HeLa cells, MKN-45 cells
Positive IHC detected in	human lung cancer tissue, human liver cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 2 publications below
IHC	See 2 publications below
IF	See 1 publications below

Product Information

12285-1-AP targets PLXDC2 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	PLXDC2 fusion protein Ag2940 相同性解析による交差性が予測される生物種
Full Name	plexin domain containing 2
Calculated molecular weight	480 aa, 54 kDa
Observed molecular weight	60 kDa
GenBank accession number	BC012885
Gene Symbol	PLXDC2
Gene ID (NCBI)	84898
RRID	AB_2166239
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q6UX71
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

PLXDC2 (Plexin-domain containing 2), also named tumor endothelial marker 7-related protein (TEM7R), is a single-pass type I membrane protein with some homology to nidogen and to plexins. PLXDC2 is located in tumor endothelium and plays a role in tumor angiogenesis. Three isoforms of PLXDC2 produced by alternative splicing have been described. Recent finding revealed that PLXDC2 is a mitogen for neural progenitors and functions as a novel regulator of the development of different brain regions, suggesting its role in coordinating proliferation and differentiation in the developing nervous system.

Protocols

Product Specific Protocols
WB protocol for PLXDC2 antibody 12285-1-AP	Download protocol
IHC protocol for PLXDC2 antibody 12285-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	IHC,IF	Front Cell Dev Biol Overexpression of PLXDC2 in Stromal Cell-Associated M2 Macrophages Is Related to EMT and the Progression of Gastric Cancer. Authors - Yiming Guan View Article
human	WB,IHC	Cancer Res Commun Adaptive c-Met-PLXDC2 Signaling Axis Mediates Cancer Stem Cell Plasticity to Confer Radioresistance-associated Aggressiveness in Head and Neck Cancer Authors - Liwei Lang View Article
human,mouse	WB	Dev Cell Secreted PTEN binds PLXDC2 on macrophages to drive antitumor immunity and tumor suppression Authors - Cheng Zhang KD Validated View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of A431 using 12285-1-AP

WB analysis using 12285-1-AP

WB analysis of HeLa using 12285-1-AP

IHC staining of human lung cancer using 12285-1-AP