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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PLXDC2 Recombinant antibody

PLXDC2 Recombinant Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

240174A11

Cat no : 83449-4-RR

Synonyms

UNQ2514/PRO6003, Tumor endothelial marker 7-related protein, TEM7R, Plexin domain-containing protein 2, plexin domain containing 2

Validation Data Gallery

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  • Western Blot (WB) analysis of MKN-45 cells using PLXDC2 Recombinant antibody (83449-4-RR)

    WB analysis of MKN-45 using 83449-4-RR

    MKN-45 cells were subjected to SDS PAGE followed by western blot with 83449-4-RR (PLXDC2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human intrahepatic cholangiocarcinoma tissue using PLXDC2 Recombinant antibody (83449-4-RR)

    IHC staining of human intrahepatic cholangiocarcinoma using 83449-4-RR

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 83449-4-RR (PLXDC2 antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human intrahepatic cholangiocarcinoma tissue using PLXDC2 Recombinant antibody (83449-4-RR)

    IHC staining of human intrahepatic cholangiocarcinoma using 83449-4-RR

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 83449-4-RR (PLXDC2 antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 83449-4-RR

    Affinity and Kinetic Characterization of 83449-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83449-4-RR against Human PLXDC2 were performed. The affinity constant is 42.5 pM.

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Tested Applications

Positive WB detected inMKN-45 cells
Positive IHC detected inhuman intrahepatic cholangiocarcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83449-4-RR is PLXDC2 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen PLXDC2 fusion protein Ag2940 相同性解析による交差性が予測される生物種
Full Name plexin domain containing 2
Calculated molecular weight 480 aa, 54 kDa
Observed molecular weight 54 kDa
GenBank accession numberBC012885
Gene symbol PLXDC2
Gene ID (NCBI) 84898
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PLXDC2 (Plexin-domain containing 2), also named tumor endothelial marker 7-related protein (TEM7R), is a single-pass type I membrane protein with some homology to nidogen and to plexins. PLXDC2 is located in tumor endothelium and plays a role in tumor angiogenesis. Three isoforms of PLXDC2 produced by alternative splicing have been described. Recent finding revealed that PLXDC2 is a mitogen for neural progenitors and functions as a novel regulator of the development of different brain regions, suggesting its role in coordinating proliferation and differentiation in the developing nervous system.

Protocols

Product Specific Protocols
WB protocol for PLXDC2 antibody 83449-4-RRDownload protocol
IHC protocol for PLXDC2 antibody 83449-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols