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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PLXNB1 Polyclonal antibody

PLXNB1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 29440-1-AP

Synonyms

KIAA0407, plexin B1, PLXN5, PLXNB1, Semaphorin receptor SEP, SEP

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PLXNB1 Polyclonal antibody (29440-1-AP)

    WB analysis using 29440-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29440-1-AP (PLXNB1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using PLXNB1 Polyclonal antibody (29440-1-AP)

    IHC staining of mouse brain using 29440-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 29440-1-AP (PLXNB1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using PLXNB1 Polyclonal antibody (29440-1-AP)

    IHC staining of mouse brain using 29440-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 29440-1-AP (PLXNB1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

29440-1-AP targets PLXNB1 in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PLXNB1 fusion protein Ag31224 相同性解析による交差性が予測される生物種
Full Name plexin B1
Calculated molecular weight 2135 aa, 232 kDa
Observed molecular weight 200-250 kDa
GenBank accession numberBC146793
Gene symbol PLXNB1
Gene ID (NCBI) 5364
RRIDAB_2918306
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

Semaphorins, originally identified as axon guidance molecules, have also been implicated in angiogenesis, immunoregulation, and cancer (PMID: 16584533). Plexins are main receptors for semaphorins and are thought to control many of the functional effects of semaphorins. PLXNB1 is one of the three plexin-B family members. It is a high-affinity receptor for semaphorin 4D (SEMA4D). Interaction of SEMA4D and PLXNB1 has important physiologic effects in the immune and nervous systems, and is also involved in tumor progression, especially in tumor angiogenesis (PMID: 20858260).

Protocols

Product Specific Protocols
WB protocol for PLXNB1 antibody 29440-1-APDownload protocol
IHC protocol for PLXNB1 antibody 29440-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols