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  • KD/KO Validated

Plexin B2 Polyclonal antibody

Plexin B2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 10602-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IHC, IF/ICC, IP, ELISA

PLXNB2, Plexin-B2, PLEXB2, Nbla00445, MM1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -


ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター(国内)に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。


保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換/補填対応を承ります。詳細はこちらをご覧ください。


Tested Applications

Positive WB detected inA549 cells, HEK-293 cells, HeLa cells, HepG2 cells, human brain tissue, MCF-7 cells, mouse liver tissue, mouse ovary tissue
Positive IP detected inmouse liver tissue
Positive IHC detected inhuman tonsillitis tissue, human liver cancer tissue, human breast cancer tissue, human gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:400-1:1600
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10602-1-AP targets Plexin B2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, chicken, goat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Plexin B2 fusion protein Ag0941 相同性解析による交差性が予測される生物種
Full Name plexin B2
Calculated molecular weight 205 kDa
Observed molecular weight 80 kDa, 240 kDa
GenBank accession numberBC004542
Gene Symbol PLXNB2
Gene ID (NCBI) 23654
RRIDAB_2166424
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO15031
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Semaphorins, originally identified as axon guidance molecules, have also been implicated in angiogenesis, immunoregulation, and cancer (PMID: 16584533). Plexins are main receptors for semaphorins and are thought to control many of the functional effects of semaphorins. PLXNB2 is one of the three plexin-B family members which participate in axon guidance and cell migration in response to semaphorins. It has been reported that plexin B2 precursor is 240 kDa and can be cleaved into alpha subunit (~170-kDa) and beta subunit (80-kDa) (PMID: 12533544). This antibody raised against 1542-1841aa of human plexin B2 can recognize precursor and beta subunit of plexin B2.

Protocols

Product Specific Protocols
WB protocol for Plexin B2 antibody 10602-1-APDownload protocol
IHC protocol for Plexin B2 antibody 10602-1-APDownload protocol
IF protocol for Plexin B2 antibody 10602-1-APDownload protocol
IP protocol for Plexin B2 antibody 10602-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
chickenIF

Nat Commun

Neuroblastoma plasticity during metastatic progression stems from the dynamics of an early sympathetic transcriptomic trajectory

Authors - Benjamin Villalard
humanWB

Nat Commun

Osteoclasts protect bone blood vessels against senescence through the angiogenin/plexin-B2 axis.

Authors - Xiaonan Liu
  • KD Validated
mouseWB,IF

EMBO J

Myeloid cells protect intestinal epithelial barrier integrity through the angiogenin/plexin-B2 axis.

Authors - Rongpan Bai
  • KD Validated
mouseIF

Hum Reprod

Whole exome sequencing in unexplained recurrent miscarriage families identified novel pathogenic genetic causes of euploid miscarriage

Authors - Xiyao Wang
humanWB,IF

J Periodontal Res

Plexin-B2 Mediates Orthodontic Tension-Induced Osteogenesis via the RhoA/F-Actin/YAP Pathway

Authors - Qiming Li
humanWB,IHC,IP

bioRxiv

Computational ranking-assisted identification of Plexin-B2 in homotypic and heterotypic clustering of circulating tumor cells in breast cancer metastasis

Authors - Emma Schuster
  • KD Validated
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