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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PLXNB2 Monoclonal antibody (67265-1-Ig)

    WB analysis using 67265-1-Ig (same clone as 67265-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67265-1-Ig (PLXNB2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67265-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of ATDC-5 cells using PLXNB2 Monoclonal antibody (67265-1-Ig)

    WB analysis of ATDC-5 using 67265-1-Ig (same clone as 67265-1-PBS)

    ATDC-5 cells were subjected to SDS PAGE followed by western blot with 67265-1-Ig (PLXNB2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67265-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of U2OS cells using PLXNB2 Monoclonal antibody (67265-1-Ig)

    WB analysis of U2OS using 67265-1-Ig (same clone as 67265-1-PBS)

    U2OS cells were subjected to SDS PAGE followed by western blot with 67265-1-Ig (PLXNB2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67265-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using PLXNB2 Monoclonal antibody (67265-1-Ig)

    WB analysis using 67265-1-Ig (same clone as 67265-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67265-1-Ig (PLXNB2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67265-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using PLXNB2 Monoclonal antibody (67265-1-Ig)

    IHC staining of mouse brain using 67265-1-Ig (same clone as 67265-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67265-1-Ig (PLXNB2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67265-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using PLXNB2 Monoclonal antibody (67265-1-Ig)

    IHC staining of mouse brain using 67265-1-Ig (same clone as 67265-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67265-1-Ig (PLXNB2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67265-1-PBS in a different storage buffer formulation.

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PLXNB2 Monoclonal antibody, PBS Only

PLXNB2 Monoclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 67265-1-PBS
Clone No.2G9B2

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, Indirect ELISA

Synonyms

dJ402G11.3, KIAA0315, MM1, Nbla00445, PLEXB2, plexin B2, PLXNB2

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67265-1-PBS targets PLXNB2 in WB, IHC, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen PLXNB2 fusion protein Ag28706 相同性解析による交差性が予測される生物種
Full Name plexin B2
Calculated molecular weight 205 kDa
Observed molecular weight 240 kDa
GenBank accession numberNM_012401
Gene Symbol PLXNB2
Gene ID (NCBI) 23654
RRIDAB_2882536
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO15031
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Semaphorins, originally identified as axon guidance molecules, have also been implicated in angiogenesis, immunoregulation, and cancer (PMID: 16584533). Plexins are main receptors for semaphorins and are thought to control many of the functional effects of semaphorins. PLXNB2 is one of the three plexin-B family members which participate in axon guidance and cell migration in response to semaphorins. It has been reported that plexin B2 precursor is 240 kDa and can be cleaved into alpha subunit (~170-kDa) and beta subunit (80-kDa) (PMID: 12533544). This antibody raised against 20-466aa of human plexin B2 can recognize precursor and alpha subunit of plexin B2.