PLZF Monoclonal antibody

Name: PLZF Monoclonal antibody
Brand: Proteintech
SKU: 66672-1-Ig

PLZF Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, rat and More (1)

Applications

WB, ELISA, IF

Conjugate

Unconjugated

Publications

CloneNo.

2E6F12

Review Product

Cat no : 66672-1-Ig

Synonyms

PLZF, ZBTB16, Zinc finger protein 145, Zinc finger protein PLZF, ZNF145

Validation Data Gallery

Filter:

AllWB

WB analysis of Ramos using 66672-1-Ig
Ramos cells were subjected to SDS PAGE followed by western blot with 66672-1-Ig (PLZF antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
WB analysis of human testis using 66672-1-Ig
human testis tissue were subjected to SDS PAGE followed by western blot with 66672-1-Ig (PLZF antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
WB analysis of Ramos using 66672-1-Ig
Ramos cells were subjected to SDS PAGE followed by western blot with 66672-1-Ig (PLZF antibody) at dilution of 1:7500 incubated at room temperature for 1.5 hours.
WB analysis of Raji using 66672-1-Ig
Raji cells were subjected to SDS PAGE followed by western blot with 66672-1-Ig (PLZF antibody) at dilution of 1:7500 incubated at room temperature for 1.5 hours.
WB analysis of Daudi using 66672-1-Ig
Daudi cells were subjected to SDS PAGE followed by western blot with 66672-1-Ig (PLZF antibody) at dilution of 1:7500 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 66672-1-Ig
HeLa cells were subjected to SDS PAGE followed by western blot with 66672-1-Ig (PLZF antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
WB analysis of THP-1 using 66672-1-Ig
THP-1 cells were subjected to SDS PAGE followed by western blot with 66672-1-Ig (PLZF antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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Cat No. 66672-1-Ig

Size/Concentration

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in

Ramos cells, Raji cells, Daudi cells, HeLa cells, THP-1 cells, human testis tissue

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 3 publications below
IF	See 1 publications below

Product Information

66672-1-Ig targets PLZF in WB, ELISA, IF applications and shows reactivity with Human, rat samples.

Tested Reactivity	Human, rat
Cited Reactivity	mouse
Host / Isotype	Mouse / IgG1
Class	Monoclonal
Type	Antibody
Immunogen	PLZF fusion protein Ag25180 相同性解析による交差性が予測される生物種
Full Name	zinc finger and BTB domain containing 16
Calculated molecular weight	673 aa, 74 kDa
Observed molecular weight	75 kDa
GenBank accession number	BC026902
Gene symbol	PLZF
Gene ID (NCBI)	7704
RRID	AB_2882026
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

PLZF, also named as Zinc finger protein 145, is a 673 amino acid protein, which belongs to the krueppel C2H2-type zinc-finger protein family. PLZF is expressed in bone marrow, early myeloid cell lines and peripheral blood mononuclear cells. PLZF may play a role in myeloid maturation and in the development and/or maintenance of other differentiated tissues.

Protocols

Product Specific Protocols
WB protocol for PLZF antibody 66672-1-Ig	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB	Front Physiol Abscopal effects of thoracic X-ray radiation on spermatogenesis in mice Authors - Pan-Pan Lai View Article
mouse	WB	iScience Chromodomain helicase DNA-binding domain 2 maintains spermatogonial self-renewal by promoting chromatin accessibility and mRNA stability Authors - Ziqian Min View Article
mouse	WB	J Hazard Mater Prenatal and postnatal exposure to polystyrene microplastics induces testis developmental disorder and affects male fertility in mice Authors - Tianxin Zhao View Article
mouse	IF	Med Microbiol Immunol Effects of acetate-producing Blautia wexlerae on oxidative stress and NLRP3 inflammasome in obesity-associated male infertility Authors - Yucheng Zhong View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PLZF Monoclonal antibody

PLZF Monoclonal Antibody for WB, ELISA

Validation Data Gallery

WB analysis of Ramos using 66672-1-Ig

WB analysis of human testis using 66672-1-Ig

WB analysis of Ramos using 66672-1-Ig

WB analysis of Raji using 66672-1-Ig

WB analysis of Daudi using 66672-1-Ig

WB analysis of HeLa using 66672-1-Ig

WB analysis of THP-1 using 66672-1-Ig

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Abscopal effects of thoracic X-ray radiation on spermatogenesis in mice

Chromodomain helicase DNA-binding domain 2 maintains spermatogonial self-renewal by promoting chromatin accessibility and mRNA stability

Prenatal and postnatal exposure to polystyrene microplastics induces testis developmental disorder and affects male fertility in mice

Effects of acetate-producing Blautia wexlerae on oxidative stress and NLRP3 inflammasome in obesity-associated male infertility