Validation Data Gallery
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Tested Applications
| Positive WB detected in | HepG2 cells, multi-tissue |
| Positive IP detected in | mouse lung tissue |
| Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 9 publications below |
| IHC | See 1 publications below |
| IF | See 2 publications below |
Product Information
10666-1-AP targets PMM2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | PMM2 fusion protein Ag1031 相同性解析による交差性が予測される生物種 |
| Full Name | phosphomannomutase 2 |
| Calculated molecular weight | 28 kDa |
| Observed molecular weight | 28-30 kDa |
| GenBank accession number | BC008310 |
| Gene Symbol | PMM2 |
| Gene ID (NCBI) | 5373 |
| RRID | AB_2166973 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O15305 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
PMM2(Phosphomannomutase 2) belongs to the eukaryotic PMM family. It is involved in the synthesis of the GDP-mannose and dolichol-phosphate-mannose required for a number of critical mannosyl transfer reactions. PMM2 catalyzes the second step in the conversion of fructose-6P to GDP-mannose.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for PMM2 antibody 10666-1-AP | Download protocol |
| IHC protocol for PMM2 antibody 10666-1-AP | Download protocol |
| IF protocol for PMM2 antibody 10666-1-AP | Download protocol |
| IP protocol for PMM2 antibody 10666-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Thromb Haemost N-Glycosylation Deficiency Reduces the Activation of Protein C and Disrupts Endothelial Barrier Integrity
| ||
J Proteome Res Changes in the striatal proteome of YAC128Q mice exhibit gene-environment interactions between mutant huntingtin and manganese. | ||
Mol Genet Metab Complex metabolic disharmony in PMM2-CDG paves the way to new therapeutic approaches | ||
J Inherit Metab Dis Lack of phosphomannomutase 2 affects Xenopus laevis morphogenesis and the non-canonical Wnt5a/Ror2 signalling. | ||
Dis Model Mech Repurposing the aldose reductase inhibitor and diabetic neuropathy drug epalrestat for the congenital disorder of glycosylation PMM2-CDG. | ||
PLoS One Heterodimerization of Two Pathological Mutants Enhances the Activity of Human Phosphomannomutase2. |