- Featured Product
- KD/KO Validated
PMP2 Polyclonal antibody
PMP2 Polyclonal Antibody for WB, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat, pig
Applications
WB, IHC, IF, ELISA
Conjugate
Unconjugated
Cat no : 12717-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | pig spinal cord tissue, human brain tissue, mouse testis tissue |
| Positive IHC detected in | human gliomas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 9 publications below |
| IHC | See 5 publications below |
| IF | See 4 publications below |
Product Information
12717-1-AP targets PMP2 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat, pig samples.
| Tested Reactivity | human, mouse, rat, pig |
| Cited Reactivity | human, mouse, rat, pig |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | PMP2 fusion protein Ag3411 相同性解析による交差性が予測される生物種 |
| Full Name | peripheral myelin protein 2 |
| Calculated molecular weight | 132 aa, 15 kDa |
| Observed molecular weight | 15-20 kDa |
| GenBank accession number | BC034997 |
| Gene symbol | PMP2 |
| Gene ID (NCBI) | 5375 |
| RRID | AB_2166978 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
PMP2 (peripheral myelin protein-2, also known as P2) is one of the major proteins of peripheral myelin and appears to be related to the transport of fatty acids or the metabolism of myelin lipids.P2 constitutes up to 15% of total protein of peripheral nervous system (PNS) myelin. It is also present in small amounts in central nervous system (CNS) myelin, being abundant in spinal cord and brain stem myelin. As a structural protein, P2 is thought to stabilize the myelin membranes.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for PMP2 antibody 12717-1-AP | Download protocol |
| IHC protocol for PMP2 antibody 12717-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Neurosci Variation among intact tissue samples reveals the core transcriptional features of human CNS cell classes. | ||
Nat Neurosci Disentangling glial diversity in peripheral nerves at single-nuclei resolution. | ||
Neuron Macrophages Expressing GALC Improve Peripheral Krabbe Disease by a Mechanism Independent of Cross-Correction. | ||
J Cell Biol Fatty acid-binding proteins 3, 7, and 8 bind cholesterol and facilitate its egress from lysosomes
| ||
Elife Conservation and divergence of myelin proteome and oligodendrocyte transcriptome profiles between humans and mice. |
