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Species Reactivity
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Conjugate
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Conjugate
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PMPCA Polyclonal antibody

PMPCA Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 26536-1-AP

Synonyms

Alpha MPP, INPP5E, KIAA0123, MPPA, P 55, PMPCA

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PMPCA Polyclonal antibody (26536-1-AP)

    WB analysis using 26536-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 26536-1-AP (PMPCA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of T-47D cells using PMPCA Polyclonal antibody (26536-1-AP)

    WB analysis of T-47D using 26536-1-AP

    T-47D cells were subjected to SDS PAGE followed by western blot with 26536-1-AP (PMPCA antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of MDA-MB-453s cells using PMPCA Polyclonal antibody (26536-1-AP)

    IP experiment of MDA-MB-453s using 26536-1-AP

    IP result of anti-PMPCA (IP:26536-1-AP, 4ug; Detection:26536-1-AP 1:500) with MDA-MB-453s cells lysate 2800ug.

  • Immunofluorescence (IF) / fluorescent staining of T-47D cells using PMPCA Polyclonal antibody (26536-1-AP)

    IF Staining of T-47D using 26536-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed T-47D cells using 26536-1-AP (PMPCA antibody), at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inMDA-MB-453s cells, T-47D cells
Positive IP detected inMDA-MB-453s cells
Positive IF/ICC detected inT-47D cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

The immunogen of 26536-1-AP is PMPCA Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PMPCA fusion protein Ag19206 相同性解析による交差性が予測される生物種
Full Name peptidase (mitochondrial processing) alpha
Calculated molecular weight 525 aa, 58 kDa
Observed molecular weight 50-58 kDa
GenBank accession numberBC132724
Gene symbol PMPCA
Gene ID (NCBI) 23203
RRIDAB_2880545
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for PMPCA antibody 26536-1-APDownload protocol
IF protocol for PMPCA antibody 26536-1-APDownload protocol
IP protocol for PMPCA antibody 26536-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nat Chem Biol

Enhanced mapping of small-molecule binding sites in cells

Authors - Jacob M Wozniak
View Article
humanWB

Hum Mol Genet

GRP75 overexpression rescues frataxin deficiency and mitochondrial phenotypes in Friedreich Ataxia cellular models.

Authors - Yi Na Dong
View Article