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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PMS1 Polyclonal antibody (28186-1-AP)

    WB analysis using 28186-1-AP (same clone as 28186-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 28186-1-AP (PMS1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28186-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using PMS1 Polyclonal antibody (28186-1-AP)

    WB analysis using 28186-1-AP (same clone as 28186-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 28186-1-AP (PMS1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28186-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of Caco-2 cells using PMS1 Polyclonal antibody (28186-1-AP)

    IF Staining of Caco-2 using 28186-1-AP (same clone as 28186-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Caco-2 cells using PMS1 antibody (28186-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 28186-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

28186-1-PBS targets PMS1 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PMS1 fusion protein Ag28132 相同性解析による交差性が予測される生物種
Full Name PMS1 postmeiotic segregation increased 1 (S. cerevisiae)
Observed molecular weight 106 kDa
GenBank accession numberBC096331
Gene Symbol PMS1
Gene ID (NCBI) 5378
RRIDAB_2881083
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP54277
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

PMS1 Homolog 1 is a member of the DNA mismatch repair enzymes (MMREs) which is to eliminate the mismatch of insertions and deletions as a consequence of DNA polymerase errors at DNA synthesis in eukaryotes. PMS1 and MLH1 could form a heterodimer MutLβ that belongs to MMREs (PMID:25619773). In the MMR system, the MutS complex recognizes the DNA mispairs firstly, and then the Mlh1-Pms1 and other associated proteins such as PCNA, RFC, ExoI were recruited, inducing Exonuclease 1 (Exo1)-dependent and -independent MMR pathways (PMID:24981171). Mutations in this gene cause hereditary nonpolyposis colorectal cancer(HNPCC), which is also known as Lynch syndrome. It was reported that PMS1 is a widely expressed protein and located in the nucleus. Alternative splicing allows for 4 isoforms of PMS1 protein.