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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PMS2 Polyclonal antibody

PMS2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 18164-1-AP

Synonyms

PMSL2, PMS2CL, PMS1 protein homolog 2, HNPCC4, EC:3.1.-.-

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PMS2 Polyclonal antibody (18164-1-AP)

    WB analysis using 18164-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 18164-1-AP (PMS2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of PC-3 cells using PMS2 Polyclonal antibody (18164-1-AP)

    WB analysis of PC-3 using 18164-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 18164-1-AP (PMS2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of PC-3 cells using PMS2 Polyclonal antibody (18164-1-AP)

    WB analysis of PC-3 using 18164-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 18164-1-AP (PMS2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human colon cancer using PMS2 Polyclonal antibody (18164-1-AP)

    IHC staining of human colon cancer using 18164-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer slide using 18164-1-AP (PMS2 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using PMS2 Polyclonal antibody (18164-1-AP)

    IHC staining of human ovary cancer using 18164-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 18164-1-AP (PMS2 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inA431 cells, PC-3 cells, Jurkat cells, MCF-7 cells, Raji cells
Positive IHC detected inhuman ovary cancer tissue, human colon cancer
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

18164-1-AP targets PMS2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PMS2 fusion protein Ag12651 相同性解析による交差性が予測される生物種
Full Name PMS2 postmeiotic segregation increased 2 (S. cerevisiae)
Calculated molecular weight 862 aa, 96 kDa
Observed molecular weight 117 kDa, 100 kDa
GenBank accession numberBC093921
Gene symbol PMS2
Gene ID (NCBI) 5395
RRIDAB_2167145
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PMS2, also named as PMSL2, belongs to the DNA mismatch repair mutL/hexB family. It is a component of the post-replicative DNA mismatch repair system (MMR). It heterodimerizes with MLH1 to form MutL alpha. MulL alpha (MLH1-PMS2) interacts physically with the clamp loader subunits of DNA polymerase III, suggesting that it may play a role to recruit the DNA polymerase III to the site of the MMR. It also implicated in DNA damage signaling, a process which induces cell cycle arrest and can lead to apoptosis in case of major DNA damages. (PMID: 16873062, PMID: 18206974) Defects in PMS2 are the cause of hereditary non-polyposis colorectal cancer type 4 (HNPCC4). Defects in PMS2 are a cause of mismatch repair cancer syndrome (MMRCS). This antibody is specific to PMS2. The calculated molecular weight of PMS2 is 96 kDa, but the modified PMS2 is about 117 kDa.(PMID: 11358834)

Protocols

Product Specific Protocols
WB protocol for PMS2 antibody 18164-1-APDownload protocol
IHC protocol for PMS2 antibody 18164-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols